References

alternative

Альтернативная энергетика и экология (ISJAEE)

Alternative Energy and Ecology (ISJAEE)

1608-8298

Международный издательский дом научной периодики "Спейс

10.15518/isjaee.2020.10.007

alternative-2151

Research Article

IV. ВОДОРОДНАЯ ЭКОНОМИКА 12. Водородная экономика

IV. HYDROGEN ECONOMY. 12. Hydrogen Economy

Автоселекция микроорганизмов избыточного активного ила, использованного в качестве инокулята при темновом получении биоводорода из субстратов с разным биополимерным составом

Auto selection of microorganisms of excess activated sludge used as an inoculum for fermentative hydrogen production from substrates with different biopolymeric composition

Литти

Ю. В.

Litti

Yu. V.

Литти Юрий Владимирович – старший научный сотрудник лаборатории микробиологии антропогенных мест обитания, кандидат биологических наук

SPIN: 1487-7611

Researcher ID: C-4945-2014

119071, г. Москва, Ленинский проспект, д.33, стр. 2

тел. +7(926)369-92-43

Yuriy V. Litti – Senior Researcher, Laboratory of Microbiology of Anthropogenic Habitats, Candidate of Biological Sciences

SPIN: 1487-7611;

Researcher ID: C-4945-2014

119071, Moscow, Leninsky prospect, 33, building 2

litty-yuriy@mail.ru

https://orcid.org/0000-0002-3603-3686

Ковалев

Д. А.

Kovalev

D. A.

Ковалев Дмитрий Александрович – заведующий лабораторией биоэнергетических технологий, кандидат технических наук

SPIN: 6513-5547;

Researcher ID: K-4810-2015

109428, г. Москва, 1-й Институтский проезд, дом

Dmitry A. Kovalev – head of the laboratory of bioenergy and supercritical technologies, candidate of technical Sciences

SPIN: 6513-5547;

Researcher ID: K-4810-2015

109428, Moscow, 1st Institutskiy proezd, building 5

https://orcid.org/0000-0002-1983-3454

Ковалев

А. А.

Kovalev

A. A.

Ковалев Андрей Александрович – старший научный сотрудник лаборатории биоэнергетических и сверхкритических технологий, кандидат технических наук

SPIN: 4267-3026;

Researcher ID: F-7045-2017;

Scopus Author ID: 57205285134

109428, г. Москва, 1-й Институтский проезд, дом 5

Andrey A. Kovalev – senior researcher of the laboratory of bioenergy and supercritical technologies, candidate of technical sciences

SPIN: 4267-3026;

Researcher ID: F-7045-2017;

Scopus Author ID: 57205285134

109428, Moscow, 1st Institutskiy proezd, building 5

https://orcid.org/0000-0002-6089-9500

Меркель

А. Ю.

Merkel

A. Yu.

Меркель Александр Юрьевич – старший научный сотрудник лаборатории разнообразия и экологии экстремофильных микроорганизмов, кандидат биологических наук

SPIN: 7479-9196;

Researcher ID: E-9427-2014;

Scopus Author ID: 55216485500

119071, г. Москва, Ленинский проспект, д.33, стр. 2

тел. +7(926)369-92-43

Alexander Y. Merkel – Laboratory of Diversity and Ecology of Extremophilic Microorganisms, Candidate of Biological Sciences

SPIN: 7479-9196;

Researcher ID: E-9427-2014;

Scopus Author ID: 55216485500

119071, Moscow, Leninsky prospect, 33, building 2

tel. +7 (926) 369-92-43

https://orcid.org/0000-0002-8619-4269

Вишнякова

А. В.

Vishnyakova

A. V.

Вишнякова Анастасия Валерьевна – младший научный сотрудник лаборатории микробиологии антропогенных мест обитания

SPIN: 4278-6659;

Researcher ID: ААK-9449-2021;

Scopus Author ID: 57216345066

119071, г. Москва, Ленинский проспект, д.33, стр. 2

тел. +7(926)369-92-43

Anastasia V. Vishnyakova – junior researcher of Laboratory of Microbiology of Anthropogenic Habitats

SPIN: 4278-6659;

Researcher ID: ААK-9449-2021;

Scopus Author ID: 57216345066

119071, Moscow, Leninsky prospect, 33, building 2

tel. +7 (926) 369-92-43

https://orcid.org/0000-0003-1118-4127

Русскова

Ю. И.

Russkova

Yu. I.

Русскова Юлия Игоревна – лаборатория микробиологии антропогенных мест обитания, младший научный сотрудник

Researcher ID: C-4945-2014;

WoS AAK-9749-2021

119071, г. Москва, Ленинский проспект, д.33, стр. 2

тел. +7(926)369-92-43

Russkova Yulia – Head of Laboratory of Microbiology of Anthropogenic Habitats, Candidate of Biological Sciences

Researcher ID: C-4945-2014;

WoS AAK-9749-2021

119071, Moscow, Leninsky prospect, 33, building 2

tel. +7 (926) 369-92-43

https://orcid.org/0000-0001-9731-3895

Ножевникова

А. Н.

Nozhevnikova

A. N.

Ножевникова Алла Николаевна – лаборатория микробиологии антропогенных мест обитания, главный научный сотрудник, доктор биологических наук

Researcher ID: D-8502-2014;

Scopus Author ID: 35516284800

119071, г. Москва, Ленинский проспект, д.33, стр. 2

тел. +7(926)369-92-43

Nozhevnikova Alla – Laboratory of Microbiology of Anthropogenic Habitats, Doctor of Science in Microbiology and Biotechnology

Researcher ID: D-8502-2014;

Scopus Author ID: 35516284800

119071, Moscow, Leninsky prospect, 33, building 2

tel. +7 (926) 369-92-43

ФИЦ Биотехнологии РАНРоссияFederal State Institution «Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of Sciences»Russian Federation

ФГБНУ ФНАЦ ВИМРоссияFederal Government Budgetary Institution of Science «Federal scientific agroengeneering centre VIM»Russian Federation

2020

02082022

028-306787

2022

Международный издательский дом научной периодики "Спейс

https://www.isjaee.com/jour/about/submissions#copyrightNotice

https://www.isjaee.com/jour/article/view/2151

При использовании смешанных органических отходов в микробном сообществе реактора темновой ферментации происходит автоселекция микроорганизмов, наиболее приспособленных к данному биополимерному составу субстрата. В этом исследовании были использованы 6 субстратов, моделирующих разные биополимеры (белки, жиры, углеводы) и их смеси, для обогащения адаптированных к данным субстратам водород-продуцирующих бактерий из необработанного активного ила. С помощью высокопроизводительного секвенирования показано, что независимо от использованного субстрата, в микробном сообществе доминировал филум Firmicutes (67-100%). При использовании субстратов, богатых углеводами, микробное разнообразие было низким и в основном представлено родами Ruminococcus (26-90%) и Thermoanaerobacterium (6-67%). Темновая ферментация жиров и белков характеризовалась большим микробным разнообразием. При использовании жиров в наибольшей степени развивались Thermoanaerobacterium (21%), Thermobrachium (19%), Tepidiphilus (16%) и Acetomicrobium (14%), при использовании белков – Thermobrachium (34%), Acetomicrobium (16%) и Clostridium sensu stricto 7 (12%). Различные микробные сообщества и субстраты приводили к различиям в характеристиках процесса и метаболических путях. Максимальным был выход водорода из крахмала и составил 138 мл/г органического вещества с 60,4 % содержанием водорода в биогазе. Доминирование рода Ruminococcus, предположительно, внесло основной вклад в производство водорода. Согласно анализу стабильных изотопов 13С небольшие количества метана при темновой ферментации белков и жиров были образованы Methanothermobacter и Methanosarcina по преимущественно водородотрофному типу метаногенеза.

When mixed organic waste is used for hydrogen production by dark fermentation, the microbial community which is most adapted to actual biopolymer composition of the substrate, is auto-selected. In this research, 6 substrates simulating different biopolymers (proteins, fats, carbohydrates) and their mixtures were used to enrich hydrogenproducing bacteria adapted to these substrates from non-pretreated activated sludge. Phylum Firmicutes dominated in the microbial community (67-100%) regardless of the substrate used, as was shown by high-throughput sequencing. Microbial diversity was low when using carbohydrate-rich substrates, and microbial community was mainly represented by Ruminococcus (26-90%) and Thermoanaerobacterium (6-67%). Dark fermentation of fats and proteins was characterized by higher microbial diversity. Thermoanaerobacterium (21%), Thermobrachium (19%), Tepidiphilus (16%) and Acetomicrobium (14%) dominated when using fats, while Thermobrachium (34%), Acetomicrobium (16%) and Clostridium sensu stricto 7 (12%) dominated when using proteins as substrate. Different microbial communities and substrates resulted in diverse process performance and metabolic pathway. Dark fermentation of starch achieved the maximum hydrogen yield of 138 ml/g volatile solids with 60.4 % hydrogen content in biogas. The dominance of genus Ruminococcus was supposed to be responsible for the highest hydrogen production. Minor amounts of methane from proteins and fats were produced by Methanothermobacter and Methanosarcina. The hydrogenotrophic pathway was slightly more predominant methane formation route than the others considered based upon to the stable 13C isotope analysis.

темновая ферментациябиоводородбиополимерымикробное разнообразиевысокопроизводительное секвенирование

dark fermentationbiohydrogenbiopolymersmicrobial diversityhigh throughput sequencing

Работа выполнена при поддержке Минобрнауки России в рамках соглашения № 075-15-2020-907 от 16.11.2020 о предоставлении гранта в форме субсидий из федерального бюджета на осуществление государственной поддержки создания и развития научного центра мирового уровня «Агротехнологии будущего».

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The authors declare that there are no conflicts of interest present.