alternativeАльтернативная энергетика и экология (ISJAEE)Alternative Energy and Ecology (ISJAEE)1608-8298Международный издательский дом научной периодики "Спейс10.15518/isjaee.2023.10.064-079alternative-2301Research ArticleI. ВОЗОБНОВЛЯЕМАЯ ЭНЕРГЕТИКА. 8. Энергокомплексы на основе ВИЭI. RENEWABLE ENERGY. 8. Energy of biomassГазовый состав в приэлектродных областях биоэлектрохимических систем на основе электрогенных свойств корнеобитаемой среды томатаGas composition in the near-electrode areas of bioelectrochemical systems based on the electrogenic properties of the tomato root environmenthttps://orcid.org/0000-0003-3802-2494КулешоваТ. Э.KuleshovaT. E.

Кулешова Татьяна Эдуардовна, к.ф.-м.н., научный сотрудник,

195220, Санкт-Петербург, Гражданский просп., д. 14.

Kuleshova Tatiana Eduardovna, Candidate of Physical and Mathematical Sciences, Researcher,

14, Grazhdansky prospekt, St. Petersburg, 195220.

www.piter.ru@bk.ruЭзеринаЕ. М.EzerinaE. M.

Эзерина Елизавета Михайловна,  аспирант, инженер,

195220, Санкт-Петербург, Гражданский просп., д. 14.

Ezerina Elizaveta Mikhailovna, Graduate Student, Engineer,

14, Grazhdansky prospekt, St. Petersburg, 195220.

https://orcid.org/0000-0002-2936-5949ВертебныйВ. Е.VertebnyV. E.

Вертебный Виталий Евгеньевич, с.н.с.,

195220, Санкт-Петербург, Гражданский просп., д. 14.

Vertebny Vitaly Evgenievich, Senior Researcher, 

14, Grazhdansky prospekt, St. Petersburg, 195220.

https://orcid.org/0000-0002-9149-3247ХомяковЮ. В.KhomyakovYu. V.

Хомяков Юрий Викторович, к.б.н., в.н.с.,

195220, Санкт-Петербург, Гражданский просп., д. 14.

Khomyakov Yuri Viktorovich, Candidate of Biological Sciences, Leading Researcher, 

14, Grazhdansky prospekt, St. Petersburg, 195220.

Федеральное государственное бюджетное научное учреждение Агрофизический научно-исследовательский институтРоссияAgrophysical Research InstituteRussian Federation2023190320240106479Copyright © Международный издательский дом научной периодики "Спейс, 20232023Международный издательский дом научной периодики "СпейсМеждународный издательский дом научной периодики "Спейсhttps://www.isjaee.com/jour/about/submissions#copyrightNoticehttps://www.isjaee.com/jour/article/view/2301

Работа посвящена комплексному исследованию свойств растительных биоэлектрохимических систем (БЭС), включая как электрогенные характеристики и мониторинг изменения газового состава приэлектродных зон, так и фотосинтетические, биохимические и морфологические параметры получаемой растительной продукции. В качестве тест-объекта выбраны томаты и три различные системы выращивания – с использованием технологии панопоники, торфяного субстрата и дерново-подзолистой супесчаной почвы. Напряжение БЭС с использованием питательного раствора составило 35-180 мВ, торфа – 160-430 мВ, почвы – 160-590 мВ в зависимости от стадии развития растений. Содержание углекислого газа в приэлектродных областях БЭС было повышено в среднем более чем в 5 раз. Обнаружено увеличение количества содержания водорода на 40% по сравнению с воздухом в БЭС на основе торфа. Идентифицировано присутствие соединений с m/z=56 и m/z=64 в газовой составляющей приэлектродных зон. Показано, что лучшими фотосинтетическими характеристиками и большей урожайности обладали томаты, выращенные в БЭС с почвой. Перспективы применения БЭС лежат в области возобновляемой энергетики, автономного автоматизированного агропроизводства и умного сельского хозяйства.

The work is devoted to a comprehensive study of the plant bioelectrochemical systems (BES) properties, including both electrogenic characteristics and monitoring of gas composition changes in the near-electrode areas, as well as photosynthetic, biochemical and morphological parameters of the resulting plant products. Tomatoes and three different growing systems were selected as a test object - using panoponics technology, peat substrate and sodpodzolic sandy loam soil. The BES voltage using a nutrient solution was 35-180 mV, peat – 160-430 mV, soil – 160- 590 mV, depending on the stage of plant development. The carbon dioxide content in the near-electrode areas of the BES was increased on average by more than 5 times. An increase in the amount of hydrogen content by 40% compared to air in peat-based BES was discovered. The presence of compounds with m/z=56 and m/z=64 in the gas component of the near-electrode areas has been identified. It was shown that tomatoes grown in BES with soil had better photosynthetic characteristics and higher yields. Prospects for the use of BES lie in the field of renewable energy, autonomous automated agricultural production and smart agriculture.

растительно-микробный топливный элементбиоэлектрогенеззеленая энергияуглекислый газводородмасс-спектрометрический анализфотосинтетическая активностьplant-microbial fuel cellbioelectrogenesisgreen energycarbon dioxidehydrogenmass spectrometric analysisphotosynthetic activityИсследования выполнены при финансовой поддержке гранта № 23-26-10050 Российского научного фонда согласно соглашению 20.04.2023 г. № 23-26- 10050 и Санкт-Петербургского научного фонда в соответствии с соглашением от 05.05.2023 г. № 23- 26-10050.References. Logan B. Microbial fuel cells / B. Logan.: John Wiley & Sons, 2008. – 199 p.. Logan B. Microbial fuel cells / B. Logan.: John Wiley & Sons, 2008. – 199 p.. McCormick A.J. Biophotovoltaics: oxygenic photosynthetic organisms in the world of bioelectrochemical systems / A.J. McCormick [et al] // Energy & Environmental Science. – 2015 – V. 8. – №. 4. – P. 1092-1109. DOI: 10.1039/C4EE03875D.. McCormick A.J. Biophotovoltaics: oxygenic photosynthetic organisms in the world of bioelectrochemical systems / A.J. McCormick [et al] // Energy & Environmental Science. – 2015 – V. 8. – №. 4. – P. 1092-1109. DOI: 10.1039/C4EE03875D.. Strik D.P. Electricity production with living plants and bacteria in a fuel cell / D.P. Strik [et al] // International Journal of Energy Research. – 2008 – V. 32. – №. 9. – P. 870-876. DOI: 10.1002/er.1397.. Strik D.P. Electricity production with living plants and bacteria in a fuel cell / D.P. Strik [et al] // International Journal of Energy Research. – 2008 – V. 32. – №. 9. – P. 870-876. DOI: 10.1002/er.1397.. Chen B.Y. Reduction of carbon dioxide emission by using microbial fuel cells during wastewater treatment / B.Y. Chen [et al] //Aerosol and Air Quality Research. – 2013 – Т. 13. – №. 1. – P. 266-274. DOI: 10.4209/aaqr.2012.05.0122.. Chen B.Y. Reduction of carbon dioxide emission by using microbial fuel cells during wastewater treatment / B.Y. Chen [et al] //Aerosol and Air Quality Research. – 2013 – Т. 13. – №. 1. – P. 266-274. DOI: 10.4209/aaqr.2012.05.0122.. Zaybak Z. Enhanced start-up of anaerobic facultatively autotrophic biocathodes in bioelectrochemical systems / Z. Zaybak [et al] //Journal of biotechnology. – 2013. – Т. 168. – №. 4. – С. 478-485. DOI: 10.1016/j.jbiotec.2013.10.001.. Zaybak Z. Enhanced start-up of anaerobic facultatively autotrophic biocathodes in bioelectrochemical systems / Z. Zaybak [et al] //Journal of biotechnology. – 2013. – Т. 168. – №. 4. – С. 478-485. DOI: 10.1016/j.jbiotec.2013.10.001.. Wang X. Sequestration of CO2 discharged from anode by algal cathode in microbial carbon capture cells (MCCs) / X. Wang [et al] //Biosensors and Bioelectronics. – 2010. – Т. 25. – №. 12. – P. 2639-2643. DOI: 10.1016/j.jbiotec.2013.10.001.. Wang X. Sequestration of CO2 discharged from anode by algal cathode in microbial carbon capture cells (MCCs) / X. Wang [et al] //Biosensors and Bioelectronics. – 2010. – Т. 25. – №. 12. – P. 2639-2643. DOI: 10.1016/j.jbiotec.2013.10.001.. Cheng S. Direct biological conversion of electrical current into methane by electromethanogenesis / S. Cheng [et al] /Environmental science & technology. – 2009. – Т. 43. – №. 10. – P. 3953-3958. DOI: 10.1021/es803531g.. Cheng S. Direct biological conversion of electrical current into methane by electromethanogenesis / S. Cheng [et al] /Environmental science & technology. – 2009. – Т. 43. – №. 10. – P. 3953-3958. DOI: 10.1021/es803531g.. Ceballos-Escalera A. Bioelectrochemical systems for energy storage: A scaled-up power-to-gas approach / A. Ceballos-Escalera [et al] //Applied energy. – 2020. – V. 260. – P. 114-138. DOI: 10.1016/j.apenergy.2019.114138.. Ceballos-Escalera A. Bioelectrochemical systems for energy storage: A scaled-up power-to-gas approach / A. Ceballos-Escalera [et al] //Applied energy. – 2020. – V. 260. – P. 114-138. DOI: 10.1016/j.apenergy.2019.114138.. Oh S.E. Hydrogen and electricity production from a food processing wastewater using fermentation and microbial fuel cell technologies / S.E. Oh, B.E. Logan //Water research. – 2005. – Т. 39. – №. 19. – С. 4673-4682. DOI: 10.1016/j.watres.2005.09.019.. Oh S.E. Hydrogen and electricity production from a food processing wastewater using fermentation and microbial fuel cell technologies / S.E. Oh, B.E. Logan //Water research. – 2005. – Т. 39. – №. 19. – С. 4673-4682. DOI: 10.1016/j.watres.2005.09.019.. Rozendal R.A. Principle and perspectives of hydrogen production through biocatalyzed electrolysis / Rozendal R.A. [et al] // International Journal of Hydrogen Energy. – 2006. –V. 31. – № 12. – P. 1632-1640. DOI: 10.1016/j.ijhydene.2005.12.006.. Rozendal R.A. Principle and perspectives of hydrogen production through biocatalyzed electrolysis / Rozendal R.A. [et al] // International Journal of Hydrogen Energy. – 2006. –V. 31. – № 12. – P. 1632-1640. DOI: 10.1016/j.ijhydene.2005.12.006.. Rozendal R.A. Efficient hydrogen peroxide generation fromorganic matter in a bioelectrochemical system / Rozendal R.A. [et al] // Electrochemistry Communications. – 2009 – V. 11. – № 9. – P. 1752–1755. DOI: 10.1016/j.elecom.2009.07.008.. Rozendal R.A. Efficient hydrogen peroxide generation fromorganic matter in a bioelectrochemical system / Rozendal R.A. [et al] // Electrochemistry Communications. – 2009 – V. 11. – № 9. – P. 1752–1755. DOI: 10.1016/j.elecom.2009.07.008.. Nevin K.P. Microbial electrosynthesis: feeding microbes electricity to convert carbon dioxide and water to multicarbon extracellular organic compounds/ K.P. Nevin [et al] // mBio – 2010 – V. 1. – № 2. DOI: 10.1128/mbio.00103-10 .. Nevin K.P. Microbial electrosynthesis: feeding microbes electricity to convert carbon dioxide and water to multicarbon extracellular organic compounds/ K.P. Nevin [et al] // mBio – 2010 – V. 1. – № 2. DOI: 10.1128/mbio.00103-10 .. Steinbusch K.J.J. Bioelectrochemical ethanol production through mediated acetate reduction by mixed cultures / K.J.J. Steinbusch [et al]// Environmental Science and Technology, – 2010. – V. 44. – № 1 – P. 513–517, DOI: 10.1021/es902371e.. Steinbusch K.J.J. Bioelectrochemical ethanol production through mediated acetate reduction by mixed cultures / K.J.J. Steinbusch [et al]// Environmental Science and Technology, – 2010. – V. 44. – № 1 – P. 513–517, DOI: 10.1021/es902371e.. Kuntke P. Ammonium recovery and energy production from urine by a microbial fuel cell / P. Kuntke // Water Research – 2012. – V. 46 – № 8 – P. 2627–2636. DOI: 10.1016/j.watres.2012.02.025.. Kuntke P. Ammonium recovery and energy production from urine by a microbial fuel cell / P. Kuntke // Water Research – 2012. – V. 46 – № 8 – P. 2627–2636. DOI: 10.1016/j.watres.2012.02.025.. Choi O. Butyrate production enhancement by Clostridium tyrobutyricum using electron mediators and a cathodic electron donor / O. Choi, Y. Um, B.-I. Sang // Biotechnology and Bioengineering. – 2012. – V. 109. – № 10. – P. 2494–2502. DOI: 10.1002/bit.24520.. Choi O. Butyrate production enhancement by Clostridium tyrobutyricum using electron mediators and a cathodic electron donor / O. Choi, Y. Um, B.-I. Sang // Biotechnology and Bioengineering. – 2012. – V. 109. – № 10. – P. 2494–2502. DOI: 10.1002/bit.24520.. van Eerten-Jansen M.C. Bioelectrochemical production of caproate and caprylate from acetate by mixed cultures / M.C. van Eerten-Jansen [et al] // Sustainable Chemistry and Engineering – 2013. – V. 1 – № 5. – P. 513–518. DOI: 10.1021/sc300168z.. van Eerten-Jansen M.C. Bioelectrochemical production of caproate and caprylate from acetate by mixed cultures / M.C. van Eerten-Jansen [et al] // Sustainable Chemistry and Engineering – 2013. – V. 1 – № 5. – P. 513–518. DOI: 10.1021/sc300168z.. Saravanan A. Microbial electrolysis cells and microbial fuel cells for biohydrogen production: Current advances and emerging challenges / A. Saravanan [et al] //Biomass Conversion and Biorefinery. – 2020. – Т. 1. – №. 5. – С. 513-518. DOI: 1-21.10.1021/sc300168z.. Saravanan A. Microbial electrolysis cells and microbial fuel cells for biohydrogen production: Current advances and emerging challenges / A. Saravanan [et al] //Biomass Conversion and Biorefinery. – 2020. – Т. 1. – №. 5. – С. 513-518. DOI: 1-21.10.1021/sc300168z.. Gupta P. Design of a microbial fuel cell and its transition to microbial electrolytic cell for hydrogen production by electrohydrogenesis / P. Gupta [et al] // Indian journal of experimental Biology. – 2013. – V. 51. – P. 860-865. DOI: nopr.niscpr.res.in/handle/123456789/21455.. Gupta P. Design of a microbial fuel cell and its transition to microbial electrolytic cell for hydrogen production by electrohydrogenesis / P. Gupta [et al] // Indian journal of experimental Biology. – 2013. – V. 51. tion / M.T. Noori, B. Min // Bioresource Technology. – 2022. DOI: 10.1016/j.biortech.2022.127641.. Sharma Y. Optimizing energy harvest in wastewater treatment by combining anaerobic hydrogen producing biofermentor (HPB) and microbial fuel cell (MFC) / Y. Sharma B. Li //International Journal of Hydrogen Energy. – 2010. – V. 35. – №. 8. – P. 3789-3797. DOI: 10.1016/j.ijhydene.2010.01.042.. Luo S. Onset Investigation on Dynamic Change of Biohythane Generation and Microbial Structure in Dual-chamber versus Single-chamber Microbial Electrolysis Cells / S. Luo [et al] // Water Res. – 2021. – V. 201. DOI: 10.1016/j.watres.2021.117326.. Logan B.E. Biologically extracting energy from wastewater: biohydrogen production and microbial fuel cells / B.E. Logan // Environ. Sci. Technol. – 2004. – Т. 38. – №. 9. – С. 160-167.. Aelterman P. Continuous electricity generation at high voltages and currents using stacked microbial fuel cells / P. Aelterman [et al] // Environ. Sci. Technol. – 2006. – Т. 40. – №. 10. – P. 3388-3394. DOI: 10.1021/es0525511.. Clemens J. Mitigation of greenhouse gas emissions by anaerobic digestion of cattle slurry / J. Clemens [et al] //Agriculture, ecosystems & environment. – 2006. – Т. 112. – №. 2-3. – С. 171-177. DOI: 10.1016/j.agee.2005.08.016.. Panova G.G. Fundamentals of physical modeling of “ideal” agroecosystems / Panova G.G. [et al] // Tech. Phys. – 2020. – V. 65. – P. 1563-1569. DOI: 10.1134/S1063784220100163.. Liu Q. Multiple syntrophic interactions drive biohythane production from waste sludge in microbial electrolysis cells / Q. Liu [et al] // Biotechnol. Biofuels. – 2016. – Т. 9. – С. 1-10. DOI: 10.1186/s13068-016-0579-x.. Panova G.G. Scientific and technical bases of year-round obtaining high yields of high-quality plant products under artificial lighting / G.G. Panova // Reports of the Russian Academy of Agricultural Sciences. – 2015 – № 4. – P. 17-21.. Noori M.T. Fundamentals and recent progress in bioelectrochemical system assisted biohythane production / M.T. Noori, B. Min // Bioresource Technology. – 2022. DOI: 10.1016/j.biortech.2022.127641.. Boitsova L.V. Biological properties, general and labile organic matter of sod-podzolic sandy loam soil when using a mineral fertilizer system / L.V. Boitsova // Agrophysics. – 2014. – V. 2. – №. 14. – P. 8-15.. Luo S. Onset Investigation on Dynamic Change of Biohythane Generation and Microbial Structure in Dual-chamber versus Single-chamber Microbial Electrolysis Cells / S. Luo [et al] // Water Res. – 2021. – V. 201. DOI: 10.1016/j.watres.2021.117326.. Kuleshova T.E. Concentration cell based on electrogenic processes in the root environment / T.E. Kuleshova [et al.] // Technical Physics Letters. – 2022. – V. 48. – № 4. – P. 66-69. DOI: 10.21883/TPL.2022.04.53176.19066.. Aelterman P. Continuous electricity generation at high voltages and currents using stacked microbial fuel cells / P. Aelterman [et al] // Environ. Sci. Technol. – 2006. – Т. 40. – №. 10. – P. 3388-3394. DOI: 10.1021/es0525511.. Kuleshova T.E. Influence of the electrode systems parameters on the electricity generation and the possibility of hydrogen production in a plant-microbial fuel cell / T.E. Kuleshova [et al.] // International Journal of Hydrogen Energy. – 2022. – № 47. – P. 24297-24309. DOI: 10.1016/j.ijhydene.2022.06.001.. Panova G.G. Fundamentals of physical modeling of “ideal” agroecosystems / Panova G.G. [et al] // Tech. Phys. – 2020. – V. 65. – P. 1563-1569. DOI: 10.1134/S1063784220100163.. Kuzmin A.G. Small-sized quadrupole mass spectrometers for the analysis of the composition of gaseous media in medicine and ecology / A.G. Kuzmin, Yu.A. Titov //Vacuum equipment and technology – 2015. – V. 25. – №. 2. – P. 35-36.. Панова Г.Г. Научно-технические основы круглогодичного получения высоких урожаев качественной растительной продукции при искусственном освещении / Г.Г. Панова // Доклады Российской академии сельскохозяйственных наук. – 2015 – № 4. – С. 17-21.. Sims D.A. Relationships between leaf pigment content and spectral reflectance across a wide range of species, leaf structures and developmental stages / D.A. Sims, J.A. Gamon // Remote sensing of environment. 2002. – V. 81. – №. 2-3. – P. 337-354. DOI: 10.1016/S0034-4257(02)00010-X.. Бойцова Л.В. Биологические свойства, общее и лабильное органическое вещество дерновоподзолистой супесчаной почвы при применении минеральной системы удобрения / Л.В. Бойцова // Агрофизика. – 2014. – Т. 2. – №. 14. – С. 8-15.. Peñuelas J. The reflectance at the 950–970 nm region as an indicator of plant water status / J. Peñuelas [et al] // International journal of remote sensing. 1993. – V. 14. – №. 10. – P. 1887-1905. DOI: 10.1080/01431169308954010.. Кулешова Т.Э. Концентрационный элемент на основе электрогенных процессов в корнеобитаемой среде / Т.Э. Кулешова [и др.] // Письма в Журнал технической физики. – 2022. – Т. 48. – № 8. – С. 29-32. DOI: 10.21883/PJTF.2022.08.52363.19066.. Gamon J.A. The photochemical reflectance index: an optical indicator of photosynthetic radiation use efficiency across species, functional types, and nutrient levels / J.A. Gamon, L. Serrano, J.S. Surfus // Physiol. Comp. Oecol. – 1997. – V. 112. – P. 492-501. DOI: 10.1007/s004420050337.. Кулешова Т.Э. Влияние параметров электродных систем на генерацию электроэнергии и возможность получения водорода в растительно-микробном топливном элементе / Т.Э. Кулешова [и др.] // Альтернативная энергетика и экология. – 2022. – № 1 – С. 32-51. DOI: 10.15518/isjaee.2022.01.032-051.. MINI-PAM Photosynthesis Yield Analyzer Manual. Edition 3. – М.: Heinz Walz GmbH, 2018. – 197 p.. Кузьмин А.Г. Малогабаритные квадрупольные масс-спектрометры для анализа состава газовых сред в медицине и экологии / А.Г. Кузьмин, Ю.А. Титов //Вакуумная техника и технология. – 2015. – Т. 25. – №. 2. – С. 35-36.. Ermakov A.I. Methods of biochemical research of plants / A.I. Ermakov. – M.: Agropromizdat, 1987. – 429 с.. Sims D.A. Relationships between leaf pigment content and spectral reflectance across a wide range of species, leaf structures and developmental stages / D.A. Sims, J.A. Gamon // Remote sensing of environment. 2002. – V. 81. – №. 2-3. – P. 337-354. DOI: 10.1016/S0034-4257(02)00010-X.. Guidelines for the determination of nitrates and nitrites in crop production. – М.: MH RSFSR, 1990. – 49 P.. Peñuelas J. The reflectance at the 950–970 nm region as an indicator of plant water status / J. Peñuelas [et al] // International journal of remote sensing. 1993. – V. 14. – №. 10. – P. 1887-1905. DOI: 10.1080/01431169308954010.. Skurikhin I.M., Tutelyan V.A. Guidelines for methods of analyzing the quality and safety of food products / I.M. Skurikhin, V.A. Tutelyan. – M.: Publishing House of Medicine, 1998. – 342 P.. Gamon J.A. The photochemical reflectance index: an optical indicator of photosynthetic radiation use efficiency across species, functional types, and nutrient levels / J.A. Gamon, L. Serrano, J.S. Surfus // Physiol. Comp. Oecol. – 1997. – V. 112. – P. 492-501. DOI: 10.1007/s004420050337.. Gamon J.A. The photochemical reflectance index: an optical indicator of photosynthetic radiation use efficiency across species, functional types, and nutrient levels / J.A. Gamon, L. Serrano, J.S. Surfus // Physiol. Comp. Oecol. – 1997. – V. 112. – P. 492-501. DOI: 10.1007/s004420050337.. MINI-PAM Photosynthesis Yield Analyzer Manual. Edition 3. – М.: Heinz Walz GmbH, 2018. – 197 p.. MINI-PAM Photosynthesis Yield Analyzer Manual. Edition 3. – М.: Heinz Walz GmbH, 2018. – 197 p.. Ермаков А.И. Методы биохимического исследования растений / А.И. Ермаков. – М.: Агропромиздат, 1987. – 429 с.. Ермаков А.И. Методы биохимического исследования растений / А.И. Ермаков. – М.: Агропромиздат, 1987. – 429 с.. Методические указания по определению нитратов и нитритов в продукции растениеводства. – М.: МЗ РСФСР, 1990. – 49 с.. Методические указания по определению нитратов и нитритов в продукции растениеводства. – М.: МЗ РСФСР, 1990. – 49 с.. Скурихин И.М., Тутельян В.А. Руководство по методам анализа качества и безопасности пищевых продуктов / И.М. Скурихин, В.А. Тутельян. – М.: Изд-во Медицина, 1998. – 342 с.. Скурихин И.М., Тутельян В.А. Руководство по методам анализа качества и безопасности пищевых продуктов / И.М. Скурихин, В.А. Тутельян. – М.: Изд-во Медицина, 1998. – 342 с.

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