References

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Альтернативная энергетика и экология (ISJAEE)

Alternative Energy and Ecology (ISJAEE)

1608-8298

Международный издательский дом научной периодики "Спейс

10.15518/isjaee.2024.07.092-120

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Research Article

I. ВОЗОБНОВЛЯЕМАЯ ЭНЕРГЕТИКА 5. Энергия биомассы

I. RENEWABLE ENERGY 5. Energy of Biomass

Оптимизация двухстадийного термофильного анаэробного сбраживания сточных вод молочного производства: влияние материала носителя на производительность процесса и микробное сообщество

Optimization of two-stage thermophilic anaerobic digestion of dairy wastewater: effect of carrier material on process performance and microbial community

Михеева

Э. Р.

Mikheeva

E. R.

Михеева Эльза Равилевна, научный сотрудник лаборатории ресурсосберегающих биотехнологий, кандидат биологических наук

Researcher ID: L-8818-2016

603950, г. Нижний Новгород, проспект Гагарина, 23

Elza R. Mikheeva, Researcher, Laboratory of Resource-Saving Biotechnology, Candidate of Biological Sciences

Researcher ID: L-8818-2016

603950, Nizhny Novgorod, Gagarina ave., 23

Катраева

И. В.

Katraeva

I. V.

Катраева Инна Валентиновна, доцент кафедры водоснабжения, водоотведения, инженерной экологии и химии, кандидат технических наук

Researcher ID: O-4715-2016

603950, г. Нижний Новгород, проспект Гагарина, 23

603950, г. Нижний Новгород, ул. Ильинская, 65

Inna V. Katraeva, associate professor of the chair of water supply, sewage, engineering ecology and chemistry, candidate of technical sciences

Researcher ID: O-4715-2016

603950, Nizhny Novgorod, Gagarina ave., 23

603950, Nizhny Novgorod, St. Ilyinskaya, 65

https://orcid.org/0000-0002-1983-3454

Ковалев

А. А.

Kovalev

A. A.

Ковалев Андрей Александрович, главный научный сотрудник лаборатории биоэнергетических технологий, доктор технических наук

Researcher ID: F-7045-2017

109428, Москва, 1-й Институтский проезд, 5

Andrey A. Kovalev, senior researcher of the laboratory of bioenergy and supercritical technologies, candidate of technical sciences

Researcher ID: F-7045-2017, Scopus Author ID: 57205285134

109428, Moscow, 1st Institutskiy Proezd, 5

https://orcid.org/0000-0002-7458-0031

Шехурдина

С. В.

Shekhurdina

S. V.

Шехурдина Светлана Витальевна, м. н. с. лаборатории микробиологии антропогенных мест обитания

Researcher ID: JZW-4863-2024, Scopus Author ID: 57564192200

119071, Москва, Ленинский пр-т, 33, 2

Svetlana V. Shekhurdina, junior researcher of Laboratory of Microbiology of Anthropogenic Habitats

Researcher ID: JZW-4863-2024, Scopus Author ID: 57564192200

119071, Moscow, Leninskiy Pr-t, 33, 2

https://orcid.org/0000-0002-5525-0459

Журавлева

Е. А.

Zhuravleva

E. A.

Журавлева Елена Александровна, м. н. с. лаборатории микробиологии антропогенных мест обитания, аспирант

Researcher ID: JBS-4297-2023, Scopus Author ID: 57216346570

119071, Москва, Ленинский пр-т, 33, 2

Elena A. Zhuravleva, junior researcher Laboratory of Microbiology of Anthropogenic Habitats, postgraduate. PhD student

Researcher ID: JBS-4297-2023, Scopus Author ID: 57216346570

119071, Moscow, Leninskiy Pr-t, 33, 2

https://orcid.org/0000-0001-8215-2814

Лайкова

А. А.

Laikova

A. A.

Лайкова Александра Алексеевна, м. н. с. лаборатории микробиологии антропогенных мест обитания

Researcher ID: IVU-7977-2023, Scopus Author ID: 58044317600

119071, Москва, Ленинский пр-т, 33, 2

Alexandra A. Laikova, junior researcher in Laboratory of Microbiology of Anthropogenic Habitats

Researcher ID: IVU-7977-2023, Scopus Author ID: 58044317600

119071, Moscow, Leninskiy Pr-t, 33, 2

https://orcid.org/0000-0002-3603-3686

Ковалев

Д. А.

Kovalev

D. A.

Ковалев Дмитрий Александрович, заведующий лабораторией биоэнергетических технологий, кандидат технических наук

Researcher ID: K-4810-2015

109428, Москва, 1-й Институтский проезд, 5

Dmitry A. Kovalev, head of the laboratory of bioenergy and supercritical technologies, candidate of technical Sciences

Researcher ID: K-4810-2015

109428, Moscow, 1st Institutskiy Proezd, 5

https://orcid.org/0000-0002-5457-4603

Литти

Ю. В.

Litti

Yu. V.

Литти Юрий Владимирович, заведующий лабораторией микробиологии антропогенных мест обитания, кандидат биологических наук

Researcher ID: C-4945-2014, Scopus Author ID: 55251689800

119071, Москва, Ленинский пр-т, 33, 2

Yuriy V. Litti, Head of Laboratory of Microbiology of Anthropogenic Habitats, Candidate of Biological Sciences

Researcher ID: C-4945-2014, Scopus Author ID: 55251689800

119071, Moscow, Leninskiy Pr-t, 33, 2

Нижегородский государственный университет имени Н. И. ЛобачевскогоРоссияLobachevsky State University of Nizhny NovgorodRussian Federation

Нижегородский государственный университет имени Н. И. Лобачевского ; Нижегородский государственный архитектурно-строительный университетРоссияLobachevsky State University of Nizhny Novgorod ; Nizhny Novgorod State University of Architecture and Civil EngineeringRussian Federation

Федеральный научный агроинженерный центр ВИМРоссияFederal Scientific Agroengineering Center VIMRussian Federation

Институт микробиологии им. Виноградского Федерального исследовательского центра «Основы биотехнологии» РАНРоссияWinogradsky Institute of Microbiology, Federal Research Center «Fundamentals of Biotechnology», of the Russian Academy of SciencesRussian Federation

2024

10082024

0792120

2024

Международный издательский дом научной периодики "Спейс

https://www.isjaee.com/jour/about/submissions#copyrightNotice

https://www.isjaee.com/jour/article/view/2451

Хотя двухстадийное анаэробное сбраживание (ДАС) не является новым процессом, информация о стабильности процесса, микробном сообществе и эффективных условиях эксплуатации ограничена и часто противоречива. В данной работе изучено влияние различных материалов-носителей (пенополиуретан, углеродный войлок, кольца Рашига и комбинация углеродного войлока и колец Рашига) на эффективность термофильного ДАС сточных вод молочных производств. Скорость загрузки органических веществ (OLR) в ацидогенном реакторе (RH) постепенно повышали с 13,74 до 32,56 г ХПК/(л∙сут) и с 0,64 до 11,46 г ХПК/(л∙сут) в метаногенных реакторах путем соответствующего снижения время гидравлического удерживания (HRT). Самая высокая скорость производства водорода 1280,3 мл/(л·сут) и выход водорода 93,2 мл/г ХПК были достигнуты при OLR 13,74 г ХПК/ (л·сут), но производство водорода прекращалось при более высоком OLR. Основными растворимыми метаболитами в RH были бутират и лактат, а в микробном сообществе доминировали Streptococcus, Thermoanaerobac- terium, Veillonellales-Selenomonadales и Pseudomonas. Наибольшая скорость образования метана (2674 мл/(л·сут) при HRT 0,5 сут) наблюдалась в реакторе с пенополиуретаном, а самый высокий выход метана (305,5 мл/г ХПК при HRT 1,5 сут) был получен в реактор, содержащий углеродный войлок. Spirochaetaceae, Desulfomicrobium, Anaerolineaceae, Candidatus Caldatribacterium и Cloacimonadaceae W5 были связаны с этими материалами и объясняли самые высокие метаногенные свойства.

Although two-stage anaerobic digestion (TSAD) is not a novel process, information of the process stability, microbial community and effective operating conditions is limited and often contradictory. In this work, the influence of different carrier materials (polyurethane foam, carbon felt, Raschig rings and a combination of carbon felt and Raschig rings) on the performance of the thermophilic TSAD of dairy wastewater was studied. The organic loading rate (OLR) in the acidogenic reactor (RH) was gradually increased from 13,74 to 32,56 g COD/(L∙d), and from 0,64 to 11,46 g COD/(L∙d) in the methanogenic reactors by correspondingly reducing the hydraulic retention time (HRT). The highest hydrogen production rate of 1280,3 ml/(L·d) and hydrogen yield of 93,2 ml/g COD were achieved at OLR of 13,74 g COD/(L·d), but hydrogen production stopped at higher OLR. The main soluble metabolites in RH were butyrate and lactate, and the microbial community was dominated by Streptococcus, Thermoanaerobacterium, Veillonellales- Selenomonadales and Pseudomonas. The highest methane production rate (2674 mL/(L·d) at HRT of 0,5 days) was observed in the reactor with polyurethane foam, while the highest methane yield (305,5 ml/g COD at HRT of 1,5 days) was obtained in a reactor containing carbon felt. Spirochaetaceae, Desulfomicrobium, Anaerolineaceae, Candidatus Caldatribacterium and Cloacimonadaceae W5 were linked to these materials and explained the highest methanogenic performance.

двухстадийное анаэробное сбраживаниемолочные сточные водынесущие материалымикробное сообщество

two-stage anaerobic digestiondairy wastewatercarrier materialsmicrobial community

Исследование выполнено на средства гранта Российского научного фонда № 21-79-10153. (https://rscf.ru/project/21-79-10153/). Е. А. Журавлева, А. А. Лайкова и Ю. В. Литти были поддержаны Министерством науки и высшего образования Российской Федерации.

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The authors declare that there are no conflicts of interest present.