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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">alternative</journal-id><journal-title-group><journal-title xml:lang="ru">Альтернативная энергетика и экология (ISJAEE)</journal-title><trans-title-group xml:lang="en"><trans-title>Alternative Energy and Ecology (ISJAEE)</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1608-8298</issn><publisher><publisher-name>Международный издательский дом научной периодики "Спейс</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.15518/isjaee.2024.11.085-092</article-id><article-id custom-type="elpub" pub-id-type="custom">alternative-2575</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>IV. ВОДОРОДНАЯ ЭКОНОМИКА 12. Водородная экономика. 12-5-12-0 Новые способы получения водорода</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>IV. HYDROGEN ECONOMY 12. Hydrogen economy. 12-5-12-0 Novel hydrogen production methods</subject></subj-group></article-categories><title-group><article-title>Влияние кратности подачи предварительно обработанного в аппарате вихревого слоя субстрата на эффективность получения биоводорода в процессе темновой ферментации</article-title><trans-title-group xml:lang="en"><trans-title>Effect of the feed rate of the substrate pre-treated in the vortex layer apparatus on the efficiency of biohydrogen production in the process of dark fermentation</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1983-3454</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ковалев</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Kovalev</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ковалев Андрей Александрович, главный научный сотрудник лаборатории биоэнергетических технологий, доктор технических наук</p><p>109428, Москва, 1-й Институтский проезд, 5</p><p>Researcher ID: F-7045-2017,Scopus Author ID: 57205285134</p></bio><bio xml:lang="en"><p>Kovalev Andrey Alexandrovich, chief researcher of the laboratory ofbioenergy technologies, doctor of technical sciences</p><p>109428, Moscow, 1-y Institutskiy proezd, 5</p><p>Researcher ID: F-7045-2017,Scopus Author ID: 57205285134</p></bio><email xlink:type="simple">kovalev_ana@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3603-3686</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ковалев</surname><given-names>Д. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Kovalev</surname><given-names>D. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ковалев Дмитрий Александрович, заведующий лабораторией биоэнергетическихтехнологий, кандидат технических наук</p><p>109428, Москва, 1-й Институтский проезд, 5</p><p>Researcher ID: K-4810-2015</p></bio><bio xml:lang="en"><p>Kovalev Dmitry Alexandrovich, head of the laboratory of bioenergy and supercritical technologies, candidate of technical sciences</p><p>109428, Moscow, 1-y Institutskiy proezd, 5</p><p>Researcher ID: K-4810-2015</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4689-843X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Панченко</surname><given-names>В. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Panchenko</surname><given-names>V. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Панченко Владимир Анатольевич, кандидат технических наук, доцент кафедры Российского университета транспорта, старший научный сотрудник лаборатории Федерального научного агроинженерного центра ВИМ</p><p>127994, Москва, ул. Образцова, д. 9</p><p>Researcher ID: P-8127-2017,Scopus Author ID: 57201922860,Web of Science Researcher ID: AAE-1758-2019</p></bio><bio xml:lang="en"><p>Panchenko Vladimir Anatolyevich, candidate of technical sciences, associate professor of theDepartment of the Russian University of Transport, senior researcher of the Laboratory of theFederal Scientific Agroengineering Center VIM</p><p>127994, Moscow, ul. Obraztsova, d. 9</p><p>Researcher ID: P-8127-2017,Scopus Author ID: 57201922860,Web of Science Researcher ID: AAE-1758-2019</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3847-2260</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Вивекананд</surname><given-names>Вивекананд</given-names></name><name name-style="western" xml:lang="en"><surname>Vivekanand</surname><given-names>Vivekanand</given-names></name></name-alternatives><bio xml:lang="ru"><p>Vivekanand Vivekanand, Assistant Professor</p><p>302017, Раджастхан, Джайпур</p><p>Scopus Author ID: 57211114758</p></bio><bio xml:lang="en"><p>Vivekanand Vivekanand, Assistant Professor</p><p>302017, Rajasthan, Jaipur</p><p>Scopus Author ID: 57211114758</p></bio><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Нидхи</surname><given-names>Парик</given-names></name><name name-style="western" xml:lang="en"><surname>Nidhi</surname><given-names>Pareek</given-names></name></name-alternatives><bio xml:lang="ru"><p>Нидхи Парик, Assistant Professor of Microbial Catalysis and Process Engineering Laboratory, Department of Microbiology</p><p>305817, Раджастхан, Аджмер</p></bio><bio xml:lang="en"><p>Nidhi Pareek, Assistant Professor of Microbial Catalysis and Process Engineering Laboratory, Department of Microbiology</p><p>305817, Rajasthan, Ajmer</p><p> </p><p> </p></bio><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шьям Кумар</surname><given-names>Масакапалли</given-names></name><name name-style="western" xml:lang="en"><surname>Shyam Kumar</surname><given-names>Masakapalli</given-names></name></name-alternatives><bio xml:lang="ru"><p>Шьям Кумар Масакапалли, Assistant Professor</p><p>175075, Химачал-Прадеш, Манди, Каманд</p><p> </p></bio><bio xml:lang="en"><p>Shyam Kumar Masakapalli, Assistant Professor</p><p>175075, Himachal Pradesh, Mandi, Kamand</p><p> </p></bio><xref ref-type="aff" rid="aff-5"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5525-0459</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Журавлева</surname><given-names>Е. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Zhuravleva</surname><given-names>E. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Журавлева Елена Александровна, мл. науч. сотр. лаборатории микробиологии антропогенных мест обитания, канд. биол. наук</p><p>119071, Москва, Ленинский пр-т, дом 33, строение 2</p><p>Researcher ID: JBS-4297-2023,Scopus Author ID: 57216346570 </p></bio><bio xml:lang="en"><p>Zhuravleva Elena Alexandrovna, junior researcher Laboratory of Microbiology of Anthropogenic Habitats, postgraduate. PhD</p><p>119071, Moscow, Leninsky Prospekt, Building 33, Building 2</p><p>Researcher ID: JBS-4297-2023,Scopus Author ID: 57216346570</p></bio><xref ref-type="aff" rid="aff-6"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8215-2814</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Лайкова</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Laikova</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Лайкова Александра Алексеевна, младший научный сотрудник лабораториимикробиологии антропогенных мест обитания, аспирант</p><p>119071, Москва, Ленинский пр-т, дом 33, строение 2</p><p>Researcher ID: IVU-7977-2023,Scopus Author ID: 58044317600</p></bio><bio xml:lang="en"><p>Laikova Alexandra Alekseevna,  junior researcher in Laboratory of Microbiology ofAnthropogenic Habitats, PhD student</p><p>119071, Moscow, Leninsky Prospekt, Building 33, Building 2</p><p>Researcher ID: IVU-7977-2023,Scopus Author ID: 58044317600</p></bio><xref ref-type="aff" rid="aff-6"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-7458-0031</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шехурдина</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Shekhurdina</surname><given-names>S. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Шехурдина Светлана Витальевна, младший научный сотрудник лабораториимикробиологии антропогенных мест обитания, аспирант</p><p>119071, Москва, Ленинский пр-т, дом 33, строение 2</p><p>Scopus Author ID: 57564192200</p></bio><bio xml:lang="en"><p>Shekhurdina Svetlana Vitalievna, junior researcher of Laboratory of Microbiology ofAnthropogenic Habitats, PhD student</p><p>119071, Moscow, Leninsky Prospekt, Building 33, Building 2</p><p>Scopus Author ID: 57564192200</p></bio><xref ref-type="aff" rid="aff-6"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0006-0931-8789</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Иваненко</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Ivanenko</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Иваненко Артем Александрович, инженер лаборатории микробиологии антропогенных мест обитания, бакалавр</p><p>119071, Москва, Ленинский пр-т, дом 33, строение 2</p><p>Researcher ID: JAX-4154-2023</p></bio><bio xml:lang="en"><p>Ivanenko Artem Alexandrovich, engineer in Laboratory of Microbiology of Anthropogenic</p><p>119071, Moscow, Leninsky Prospekt, Building 33, Building 2 Habitats, bachelor</p><p>Researcher ID: JAX-4154-2023</p></bio><xref ref-type="aff" rid="aff-6"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5457-4603</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Литти</surname><given-names>Ю. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Litti</surname><given-names>Yu. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Литти Юрий Владимирович, заведующий лабораторией микробиологииантропогенных мест обитания, кандидат биологических наук</p><p>119071, Москва, Ленинский пр-т, дом 33, строение 2</p><p>Researcher ID: C-4945-2014,Scopus Author ID: 55251689800</p></bio><bio xml:lang="en"><p>Litti Yuri Vladimirovich, Head of Laboratory of Microbiology of AnthropogenicHabitats, Candidate of Biological Sciences</p><p>119071, Moscow, Leninsky Prospekt, Building 33, Building 2</p><p>Researcher ID: C-4945-2014,Scopus Author ID: 55251689800</p></bio><xref ref-type="aff" rid="aff-6"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Федеральное государственное бюджетное научное учреждение «Федеральный научный агроинженерный центр ВИМ»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal State Budgetary Scientific Institution</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Российский университет транспорта</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Russian University of Transport</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Центр энергетики и окружающей среды, Национальный технологический институт Малавии</institution><country>Индия</country></aff><aff xml:lang="en"><institution>Center for Energy and Environment, Malaviya National Institute of Technology</institution><country>India</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>Школа естественных наук, Центральный университет Раджастхана</institution><country>Индия</country></aff><aff xml:lang="en"><institution>School of Life Sciences, Central University of Rajasthan</institution><country>India</country></aff></aff-alternatives><aff-alternatives id="aff-5"><aff xml:lang="ru"><institution>Центр BioX, Школа фундаментальных наук, Индийский технологический институт</institution><country>Индия</country></aff><aff xml:lang="en"><institution>BioX Center, School of Basic Sciences, Indian Institute of Technology</institution><country>India</country></aff></aff-alternatives><aff-alternatives id="aff-6"><aff xml:lang="ru"><institution>Институт микробиологии им. С. Н. Виноградского, Федеральный исследовательский центр «Фундаментальные основы биотехнологии» Российской академии наук</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Institute of Microbiology named after S. N. Vinogradsky, Federal Research Center&#13;
«Fundamentals of Biotechnology» of the Russian Academy of Sciences</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2024</year></pub-date><pub-date pub-type="epub"><day>14</day><month>03</month><year>2025</year></pub-date><volume>0</volume><issue>11</issue><fpage>85</fpage><lpage>92</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Международный издательский дом научной периодики "Спейс, 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Международный издательский дом научной периодики "Спейс</copyright-holder><copyright-holder xml:lang="en">Международный издательский дом научной периодики "Спейс</copyright-holder><license xlink:href="https://www.isjaee.com/jour/about/submissions#copyrightNotice" xlink:type="simple"><license-p>https://www.isjaee.com/jour/about/submissions#copyrightNotice</license-p></license></permissions><self-uri xlink:href="https://www.isjaee.com/jour/article/view/2575">https://www.isjaee.com/jour/article/view/2575</self-uri><abstract><p>Для утилизации пищевых отходов, количество которых увеличивается с каждым годом, в рамках проекта РНФ № 22-49-02002 (<ext-link xlink:href="https://rscf.ru/project/22-49-02002/" ext-link-type="uri">https://rscf.ru/project/22-49-02002/</ext-link>) была разработана экспериментальная установка для темновой ферментации органических отходов. Данное исследование направлено на оценку влияния кратности подачи предобработанного в АВС субстрата на эффективность получения биоводорода в процессе темновой ферментации. Согласно полученным данным, кратность подачи предобработанного в АВС субстрата в реактор темновой ферментации имеет существенное влияние на динамику образования биоводорода из органического вещества: снижение кратности подачи приводило к уменьшению амплитуды при увеличении среднего значения объемного выхода биоводорода (на 18% до 0,62 л/(л • ч) при снижении кратности в 3 раза). Таким образом увеличение частоты подачи субстрата позволяет снизить суточные колебания выхода биоводорода при одновременной интенсификации процесса темновой ферментации.</p></abstract><trans-abstract xml:lang="en"><p>In order to utilize food waste, the amount of which increases every year, an experimental setup for dark fermentation of organic waste was developed within the framework of the RSF project No. 22-49-02002 (<ext-link xlink:href="https://rscf" ext-link-type="uri">https://rscf</ext-link>. ru/project/22-49-02002/). This study is aimed at assessing the effect of the feed rate of the substrate pre-treated in the ABC on the efficiency of biohydrogen production during dark fermentation. According to the data obtained, the feed rate of the substrate pre-treated in the ABC to the dark fermentation reactor has a significant effect on the dynamics of biohydrogen formation from organic matter: a decrease in the feed rate led to a decrease in the amplitude with an increase in the average value of the volumetric yield of biohydrogen (by 18% to 0,62 l / (l h) with a decrease in the feed rate by 3 times). Thus, an increase in the substrate feed frequency makes it possible to reduce daily fluctuations in the biohydrogen yield while simultaneously intensifying the dark fermentation process.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>анаэробная биоконверсия</kwd><kwd>биоводород</kwd><kwd>темновая ферментация</kwd><kwd>кратность подачи субстрата</kwd><kwd>аппарат вихревого слоя</kwd><kwd>предобработка</kwd><kwd>пищевые отходы</kwd></kwd-group><kwd-group xml:lang="en"><kwd>anaerobic bioconversion</kwd><kwd>biohydrogen</kwd><kwd>dark fermentation</kwd><kwd>substrate feed rate</kwd><kwd>vortex layer apparatus</kwd><kwd>pretreatment</kwd><kwd>food waste</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Исследование выполнено за счет гранта Российского научного фонда № 22-49-02002, https://rscf.ru/ project/22-49-02002/</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Abu Hatab A., Cavinato M. 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