References

alternative

Альтернативная энергетика и экология (ISJAEE)

Alternative Energy and Ecology (ISJAEE)

1608-8298

Международный издательский дом научной периодики "Спейс

10.15518/isjaee.2025.06.029-062

alternative-2663

Research Article

IV. ВОДОРОДНАЯ ЭКОНОМИКА 12. Водородная экономика. 12-5-12-0 Новые способы получения водорода

IV. HYDROGEN ECONOMY 12. Hydrogen economy. 12-5-12-0 Novel hydrogen production methods

Оптимизация непрерывного двухстадийного производства биогитана из сточных вод кондитерской промышленности

Optimization of continuous two-stage production of biogitan from wastewater of the confectionery industry

https://orcid.org/0000-0001-8659-3166

Михеева

Э. Р.

Mikheeva

E. R.

Михеева Эльза Равилевна - научный сотрудник лаборатории ресурсосберегающих биотехнологий, кандидат биологических наук.

Образование: Нижегородский государственный технический университет им. Р. Е. Алексеева (НГТУ), 2009 г., химическая технология и биотехнология.

Область научных интересов: биогаз; метановодородный газ; осадки сточных вод; анаэробная ферментация; биотехнология; метаногенные бактерии; отходы пищевой промышленности; аппарат вихревого слоя; микроскопия; клетки крови; квантовые точки; нейтрофильные гранулоциты.

Публикации: более 50

Индекс Хирша (РИНЦ): 7 Researcher ID: L-8818-2016 РИНЦ: 607913

603022, г. Нижний Новгород, проспект Гагарина 23, корпус 5

Mikheeva Elsa Ravilevna - Researcher, Laboratory of Resource-Saving Biotechnology, Candidate of Biological Sciences.

Education: Nizhny Novgorod State Technical University n. a. R. E. Alekseev, 2009, chemical technology and biotechnology.

Area of scientific interests: biogas; biohythane; sewage sludge; anaerobic fermentation; biotechnology; methanogenic bacteria; food waste; vortex layer apparatus; microscopy; blood cells; quantum dots; neutrophilic granulocytes.

Publications: more 50

603022, Nizhny Novgorod, Gagarin Avenue 23, Building 5

https://orcid.org/0000-0002-7458-0031

Шехурдина

С. В.

Shekhurdina

S. V.

Шехурдина Светлана Витальевна - младший научный сотрудник лаборатории микробиологии антропогенных мест обитания, аспирант.

Образование: Московский Государственный Университет имени М. В. Ломоносова (МГУ), 2023 г., микробиолог.

Область научных интересов: анаэробное сбраживание; прямой межвидовой перенос электронов; анаэробные микроорганизмы; метаногенные сообщества; метаногенез; биогаз; анаэробная переработка органических отходов.

Публикации: более 15

Индекс Хирша: Scopus – 7; WoS – 7 Scopus Author ID: 57564192200

119071, г. Москва, Ленинский проспект, д. 33, стр. 2

Shekhurdina Svetlana Vitalievna - junior researcher of Laboratory of Microbiology of Anthropogenic Habitats, PhD student

Education: Lomonosov Moscow State University (MSU), 2021, Microbiologist.

Area of scientific interests: anaerobic digestion; direct interspecies electron transfer (DIET); anaerobic microorganisms; methanogenic communities; methanogenesis; biogas; anaerobic processing of organic waste.

Publications: more 15

119071, Moscow, Leninsky Prospekt, 33, Building 2

https://orcid.org/0000-0002-5951-0430

Катраева

И. В.

Katraeva

I. V.

Катраева Инна Валентиновна - кандидат технических наук, доцент кафедры водоснабжения, водоотведения, инженерной экологии и химии; научный сотрудник лаборатории ресурсосберегающих биотехнологий .

Образование: Нижегородский государственный архитектурно-строительный университет, 1991 г., инженер.

Область научных интересов: анаэробные микроорганизмы; очистка сточных вод; анаэробно-биохимическая очистка; анаэробная переработка отходов животноводства; возобновляемые источники энергии.

Публикации: 134

Индекс Хирша: РИНЦ – 8; Scopus – 7 Scopus Author ID: 57191839730

603950, г. Нижний Новгород, ул. Ильинская, д. 65

Katraeva Inna Valentinovna - candidate of technical sciences, Associate Professor of the department of water supply, sanitation, engineering ecology and chemistry; researcher at the laboratory of Resource-Saving Biotechnologies.

Education: Nizhny Novgorod State University of Architecture and Civil Engineering, 1991, engineer.

Area of scientific interests: anaerobic microorganisms; waste-water treatment; anaerobic-biochemical treatment; anaerobic processing of animal waste; renewable energy sources.

Publications: 134

603950, Nizhny Novgorod, Ilyinskaya Street, 65

https://orcid.org/0000-0002-1983-3454

Ковалев

А. А.

Kovalev

A. A.

Ковалев Андрей Александрович - главный научный сотрудник лаборатории биоэнергетических технологий, доктор технических наук.

Образование: Московский государственный университет путей сообщения (МИИТ), 2009 г., инженер.

Область научных интересов: возобновляемые источники энергии; анаэробная переработка отходов животноводства; производство биогаза из биомассы; теплоэнергетические установки; тепломассообмен.

Публикации: более 200

Индекс Хирша: РИНЦ – 18; Scopus – 18; Researchgate – 18 Researcher ID: F-7045-2017 Scopus Author ID: 57205285134

https://www.researchgate.net/profile/Andrey-Kovalev-8

109428, г. Москва, 1-й Институтский проезд, дом 5

+79263477955

Kovalev Andrey Alexandrovich - chief researcher of the laboratory of bioenergy technologies, doctor of technical sciences.

Education: Moscow state University of railway engineering (MIIT), 2009, engineer.

Area of scientific interests: renewable energy; anaerobic digestion of animal waste; biogas production from biomass heat and power plants; heat and mass transfer.

Publications: more 200

109428, Moscow, 1st Institutsky Proyezd, 5

+79263477955

kovalev_ana@mail.ru

https://orcid.org/0000-0002-3603-3686

Ковалев

Д. А.

Kovalev

D. A.

Ковалев Дмитрий Александрович - заведующий лабораторией биоэнергетических технологий, кандидат технических наук.

Образование: Московский государственный индустриальный университет (МГИУ), 2003 г., инженер.

Область научных интересов: возобновляемые источники энергии; анаэробная переработка отходов животноводства; технические инновации в сельском хозяйстве и защите окружающей среды; производство биогаза из биомассы.

Публикации: более 200

Индекс Хирша РИНЦ – 17; Scopus – 11; WoS – 8

Researcher ID: K-4810-2015

109428, г. Москва, 1-й Институтский проезд, дом 5

Kovalev Dmitry Alexandrovich - head of the laboratory of bioenergy and supercritical technologies, candidate of technical sciences.

Education: Moscow state industrial University (MSIU), 2003, engineer.

Area of scientific interests: renewable energy; anaerobic digestion of animal waste; technical innovations in agriculture and environmental protection; the production of biogas from biomass.

Publications: more 200

109428,Moscow, 1st Institutsky Proyezd, 5

https://orcid.org/0000-0002-5525-0459

Журавлева

Е. А.

Zhuravleva

E. A.

Журавлева Елена Александровна - науч. сотр. лаборатории микробиологии антропогенных мест обитания, канд. биол. наук.

Образование: Московский государственный университет имени М. В. Ломоносова, 2019 г., микробиолог.

Область научных интересов: анаэробные микроорганизмы; метаногенные сообщества микроорганизмов; метаногенез; органические отходы; очистка сточных вод; прямой межвидовой перенос электронов; синтрофия.

Публикации: более 30

Индекс Хирша: РИНЦ – 7; Scopus – 13; WoS – 12 Researcher ID: JBS-4297-2023 Scopus Author ID: 57216346570

119071, г. Москва, Ленинский проспект, д. 33, стр. 2

Zhuravleva Elena Alexandrovna - junior researcher Laboratory of Microbiology of Anthropogenic Habitats, postgraduate. PhD

Education: Lomonosov Moscow State University, 2019, microbiologist.

Area of scientific interests: anaerobic microorganisms; methanogenic communities of microorganisms; methanogenesis; organic waste; wastewater treatment; direct interspecies electron transfer; syntrophy.

Publications: more 30

119071, Moscow, Leninsky Prospekt, 33, Building 2

https://orcid.org/0000-0001-8215-2814

Лайкова

А. А.

Laikova

A. A.

Лайкова Александра Алексеевна - младший научный сотрудник лаборатории микробиологии антропогенных мест обитания, аспирант.

Образование: Московский Государственный Университет имени М. В. Ломоносова, 2023 г., микробиолог.

Область научных интересов: биоводород; биогаз; биогитан; осадки сточных вод; анаэробная ферментация; биотехнология; анаэробная переработка отходов; водородпродуцирующие бактерии; метаногенное сообщество.

Публикации: более 15

Индекс Хирша: РИНЦ – 6; Scopus – 7; WoS – 7 Researcher ID: IVU-7977-2023

Scopus Author ID: 58044317600

119071, г. Москва, Ленинский проспект, д. 33, стр. 2

Laikova Alexandra Alekseevna - junior researcher in Laboratory of Microbiology of Anthropogenic Habitats, PhD student.

Education: M. V. Lomonosov Moscow State University, 2021, Microbiologist.

Area of scientific interests: biohydrogen; biogas; biohythane; sewage sludge; anaerobic fermentation; biotechnology; anaerobic conversion of waste; hydrogen-producing bacteria; methanogenic community.

Publications: more 15

119071, Moscow, Leninsky Prospekt, 33, Building 2

https://orcid.org/0009-0006-0931-8789

Иваненко

А. А.

Ivanenko

A. A.

Иваненко Артем Александрович - инженер лаборатории микробиологии антропогенных мест обитания, магистр

Образование: Московский Государственный Университет имени М. В. Ломоносова, 2024 г., экология и природопользование.

Область научных интересов: биогаз; биоводород; анаэробная переработка отходов; биотехнология; метаногенные сообщества микроорганизмов.

Публикации: более 5

Индекс Хирша: Scopus – 3

Researcher ID: JAX-4154-2023

119071, г. Москва, Ленинский проспект, д. 33, стр. 2

119899, г. Москва, Ленинские Горы, д. 1, стр. 12

Ivanenko Artem Alexandrovich - engineer in Laboratory of Microbiology of Anthropogenic Habitats, bachelor.

Education: M. V. Lomonosov State University, 2024, ecology and environmental management.

Area of scientific interests: biogas, biohydrogen; anaerobic conversion of waste; biotechnology; methanogenic community; biorefinery.

Publications: more 5

119071, Moscow, Leninsky Prospekt, 33, Building 2

119899, Moscow, Leninskie Gory, 1, Building 12

https://orcid.org/0000-0002-4689-843X

Панченко

В. А.

Panchenko

V. A.

Панченко Владимир Анатольевич - кандидат технических наук, доцент кафедры; старший научный сотрудник лаборатории

Образование: Московский государственный технический университет имени Н. Э. Баумана, 2009 г., инженер.

Область научных интересов: возобновляемая энергетика; трёхмерное моделирование; электротранспорт; циклическая экономика и устойчивое развитие.

Публикации: 300

Индекс Хирша: РИНЦ – 20; Scopus: – 7; WoS: – 10 Researcher ID: P-8127-2017

Scopus Author ID: 57201922860

Web of Science Researcher ID: AAE-1758-2019

127994, г. Москва, ул. Образцова, д. 9, стр. 9

109428, г. Москва, 1-й Институтский проезд, дом 5

Panchenko Vladimir Anatolyevich - candidate of technical sciences, associate professor of the Department; senior researcher of the Laboratory

Education: Moscow State Technical University named after N. E. Bauman. 2009, engineer.

Area of scientific interests: renewable energy; 3D modeling; electric transport; circular economy and sustainable development.

Publications: 300

127994, Moscow, Obraztsova Street, 9, Building 9

https://orcid.org/0000-0002-5457-4603

Литти

Ю. В.

Litti

Yu. V.

Литти Юрий Владимирович - заведующий лабораторией микробиологии антропогенных мест обитания, кандидат биологических наук.

Образование: Российский химико-технологический университет им. Д. И. Менделеева (РХТУ), 2008 г., инженер.

Область научных интересов: анаэробные микроорганизмы; метаногенные сообщества микроорганизмов; метаногенез; твердые органические отходы; очистка сточных вод; нитрификация; денитрификация; процесс анаммокс; анаммокс-бактерии.

Публикации: более 90

Индекс Хирша: РИНЦ – 20; Scopus – 21; WoS – 19 Researcher ID: C-4945-2014 Scopus Author ID: 55251689800

119071, г. Москва, Ленинский проспект, д. 33, стр. 2

Litti Yuri Vladimirovich - Head of Laboratory of Microbiology of Anthropogenic Habitats, Candidate of Biological Sciences.

Education: D. Mendeleev University of Chemical Technology of Russia (MUCTR) 2008, engineer.

Area of scientific interests: anaerobic microorganisms; methanogenic communities of microorganisms; methanogenesis; solid organic waste; wastewater treatment; nitrification; denitrification; anammox process; anammox bacteria.

Publications: more 90

119071, Moscow, Leninsky Prospekt, 33, Building 2

Нижегородский государственный университет имени Н. И. ЛобачевскогоРоссияNizhny Novgorod State University named after N. I. LobachevskyRussian Federation

ФИЦ Биотехнологии РАНРоссияFederal Research Center of Biotechnology of the Russian Academy of SciencesRussian Federation

ФГБОУ ВО ННГАСУ; НИИ химии Национального исследовательского Нижегородского государственного университета им. Н. И. ЛобачевскогоРоссияNizhny Novgorod State University of Architecture and Civil Engineering; Research Institute of Chemistry of National Research Nizhny Novgorod State University N. I. LobachevskyRussian Federation

ФГБНУ ФНАЦ ВИМРоссияFederal Research Center of Virology and MicrobiologyRussian Federation

ФИЦ Биотехнологии РАН; Московский государственный университет имени М. В. Ломоносова, Биологический факультетРоссияFederal Research Center of Biotechnology of the Russian Academy of Sciences; Lomonosov Moscow State University, Faculty of BiologyRussian Federation

Российский университет транспорта; ФГБНУ ФНАЦ ВИМРоссияRussian University of Transport; Federal Scientific Agroengineering Center VIM.Russian Federation

2025

24082025

062962

2025

Международный издательский дом научной периодики "Спейс

https://www.isjaee.com/jour/about/submissions#copyrightNotice

https://www.isjaee.com/jour/article/view/2663

Двухстадийная анаэробная ферментация представляет собой перспективный метод получения смеси Н2 и СН4 (биогитана) из различных органических отходов. Однако для масштабирования этого процесса требуется оптимизация условий, чтобы максимизировать удельный выход и скорость образования газообразных продуктов, а также обеспечить оптимальное соотношение Н2 и СН4 в биогитане. В данной работе варьировали гидравлическое время удержания (HRT) и нагрузку по органическому веществу (OLR) в мезофильном кислотогенном (RH) и термофильном метаногенном (R) реакторах с целью оптимизации непрерывного двухстадийного производства биогитана из сточных вод кондитерской промышленности. Удельный выход Н2 (87,8 ± 20,3 мл/г ХПК) и скорость образования Н2 (2352 ± 376 мл/(л∙сут)) были максимальными при OLR в RH, равном 26,8 г ХПК/(л·сут). Удельный выход СН4 (286,3 ± 29,1 мл/г ХПК) был максимален при HRT и OLR в R, равным 2 сут и 2,05 г ХПК/(л·сут), соответственно, а скорость образования СН4 (964,6 ± 162 мл/(л·сут)) была максимальна при HRT и OLR в R, равным 2 сут и 4,6 г ХПК/(л·сут), соответственно. Оптимальный состав биогитана (10% Н2 , 50,4% СН4 , 39,6% СО2 ) был получен, когда RH функционировал при OLR, равным 12,8 г ХПК/(л·сут), а R при HRT и OLR, равным 3 сут и 1,37 г ХПК/(л·сут), соответственно. В оптимальных режимах работы в Н2-продуцирующем микробном сообществе RH доминировали Caproiciproducens и Clostridium sensu stricto 12, а в R – синтрофные бактерии Cloacimonadaceae W5, Lentimicrobium, Anaerolinea, и гидрогенотрофные метаногены Methanothermobacter. Полученные результаты способствуют дальнейшему совершенствованию двухстадийной анаэробной ферментации для производства биогитана из концентрированных сточных вод.

Two-stage anaerobic fermentation is a promising method for producing a mixture of H2 and CH4 (biogas) from various organic waste materials. However, scaling up this process requires optimization of the conditions to maximize the specific yield and rate of gas production, as well as to ensure an optimal ratio of H2 and CH4 in the biogas. In this work, the hydraulic retention time (HRT) and organic load rate (OLR) were varied in the mesophilic acidogenic (RH) and thermophilic methanogenic (R) reactors in order to optimize the continuous two-stage production of biogitan from the wastewater of the confectionery industry. The specific yield of H2 (87,8 ± 20,3 ml/g COD) and the rate of H2 formation (2352 ± 376 ml/(l∙day)) were maximum at an OLR in RH equal to 26,8 g COD/(l∙day). The specific yield of CH4 (286,3 ± 29,1 ml/g COD) was maximum at HRT and OLR in R equal to 2 days and 2,05 g COD/(l·day), respectively, and the rate of CH4 formation (964,6 ± 162 ml/(l·day)) was maximum at HRT and OLR in Requal to 2 days and 4,6 g COD/(l·day), respectively. The optimal composition of biogitan (10% H2, 50,4% CH4 , 39,6% CO2 ) was obtained when RH was operated at an OLR of 12,8 g BOD/(l·day), and R was operated at an HRT of 3 days and an OLR of 1,37 g BOD/(l·day), respectively. In the optimal operating modes, the H2-producing microbial community RH was dominated by Caproiciproducens and Clostridium sensu stricto 12, while R was dominated by syntrophic bacteria Cloacimonadaceae W5, Lentimicrobium, Anaerolinea, and hydrogenotrophic methanogens Methanothermobacter. These results contribute to the further improvement of two-stage anaerobic fermentation for the production of biogas from concentrated wastewater.

биоводородбиометанбиогитандвухстадийная анаэробная ферментациясточные воды кондитерской промышленности

biohydrogenbiomethanebiogitantwo-stage anaerobic fermentationwastewater from the confectionery industry

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The authors declare that there are no conflicts of interest present.