alternativeАльтернативная энергетика и экология (ISJAEE)Alternative Energy and Ecology (ISJAEE)1608-8298Международный издательский дом научной периодики "Спейс10.15518/isjaee.2025.12.039-106alternative-2727Research ArticleIV. ВОДОРОДНАЯ ЭКОНОМИКА. 12. Водородная экономика. 12-5-12-0 Новые способы получения водорода. 12-5-12-0 Топливные элементыIV. HYDROGEN ECONOMY. 12. Hydrogen economy. 12-5-12-0 Hydrogen production methods. 12-5-12-0 Novel hydrogen production methodsУвеличение светонезависимой выработки биоводорода путем стимуляции активности гидрогеназ: критический обзорEnhancement of light-independent biohydrogen production through stimulation of hydrogenase activity: a critical reviewhttps://orcid.org/0000-0001-8215-2814ЛайковаА. А.LaikovaA. A.

Лайкова Александра Алексеевна, младший научный сотрудник лаборатории микробиологии антропогенных мест обитания, аспирант,

119071, г. Москва, Ленинский пр-т, д. 33, строение 2.

ResearcherID: IVU-7977-2023;

Scopus AuthorID: 58044317600.

Laikova Alexandra Alekseevna, Junior Researcher in Laboratory of Microbiology of Anthropogenic Habitats, PhD Student,

119071, Moscow, Leninsky Prospekt, 33, Building 2.

Researcher ID: IVU-7977-2023; Scopus Author ID: 58044317600.

https://orcid.org/0000-0002-5525-0459ЖуравлеваЕ. А.ZhuravlevaE. A.

Журавлева Елена Александровна, к. б. н., научный сотрудник лаборатории микробиологии антропогенных мест обитания,

119071, г. Москва, Ленинский пр-т, д. 33, строение 2.

Researcher ID: JBS-4297-2023;

Scopus Author ID: 57216346570.

Zhuravleva Elena Alexandrovna, PhD, Researcher in Laboratory of Microbiology of Anthropogenic Habitats, 

119071, Moscow, Leninsky Prospekt, 33, Building 2.

Researcher ID: JBS-4297-2023; Scopus Author ID: 57216346570.

https://orcid.org/0009-0006-0931-8789ИваненкоА. А.IvanenkoA. A.

Иваненко Артем Александрович, младший научный сотрудник лаборатории микробиологии антропогенных мест обитания,

119071, г. Москва, Ленинский пр-т, д. 33, строение 2;

119899, г. Москва, Ленинские Горы, д. 1, строение 12.

Researcher ID: JAX-4154-2023;

Scopus Author ID: 57195447250.

Ivanenko Artem Aleksandrovich, Junior Researcher in Laboratory of Microbiology of Anthropogenic Habitats,

119071, Moscow, Leninsky Prospekt, 33, Building 2;

119899, Moscow, Leninskie Gory, 1, Building 12.

Researcher ID: JAX-4154-2023; Scopus Author ID: 57195447250

https://orcid.org/0000-0002-7458-0031ШехурдинаС. В.ShekhurdinaS. V.

Шехурдина Светлана Витальевна, младший научный сотрудник лаборатории микробиологии антропогенных мест обитания, аспирант,

119071, г. Москва, Ленинский пр-т, д. 33, строение 2.

Scopus Author ID: 57564192200;

Researcher ID: JZW-4863-2024.

Shekhurdina Svetlana Vitalievna, Junior Researcher of Laboratory of Microbiology of Anthropogenic Habitats, PhD Student,

119071, Moscow, Leninsky Prospekt, 33, Building 2.

Scopus Author ID: 57564192200; Researcher ID: JZW-4863-2024.

https://orcid.org/0000-0002-1983-3454КовалевА. А.KovalevA. A.

Ковалев Андрей Александрович, доктор технических наук, главный научный сотрудник лаборатории биоэнергетических технологий,

109428, г. Москва, 1-й Институтский проезд, 5.

Researcher ID: F-7045-2017;

Scopus Author ID: 57205285134.

https://www.researchgate.net/profile/Andrey-Kovalev-8

Phone: +7926347795

Kovalev Andrey Alexandrovich, Doctor of Technical Sciences, Senior Researcher of the Laboratory of Bioenergy and Supercritical Technologies,

109428, Moscow, 1st Institutsky Proyezd, 5.

Researcher ID: F-7045-2017; Scopus Author ID: 57205285134.

https://www.researchgate.net/profile/Andrey-Kovalev-8

kovalev_ana@mail.ruhttps://orcid.org/0000-0002-3603-3686КовалевД. А.KovalevD. A.

Ковалев Дмитрий Александрович, заведующий лабораторией биоэнергетических технологий, кандидат технических наук,

109428, г. Москва, 1-й Институтский проезд, д. 5.

Researcher ID: K-4810-2015.

Kovalev Dmitry Alexandrovich, Head of the Laboratory of Bioenergy and Supercritical Technologies, Candidate of Technical Sciences

109428, Moscow, 1st Institutsky Proyezd, 5.

Researcher ID: K-4810-2015.

https://orcid.org/0000-0003-3474-1555ПиллаиСантошPillaiSanthosh

Сантош Пиллаи,  кандидат наук, профессор университета, 

Дурбан.

Scopus Author ID: 57194010507.

Santhosh Pillai, PhD,

Durban.

Scopus Author ID: 57194010507.

https://orcid.org/0000-0001-7558-7748де КарвальюХулио СезарDe CarvalhoJulio Cesar

Хулио Сезар де Карвалью, доктор философии в области биотехнологических процессов, профессор инженерии,

Куритиба.

Scopus Author ID: 9244670900.

Julio Cesar de Carvalho, PhD, Professor of Bioprocess Engineering,

Curitiba.

Scopus Author ID: 9244670900

https://orcid.org/0000-0001-7630-6864СокколКарлос РикардоSoccolCarlos Ricardo

Карлос Рикардо Соккол,  руководитель исследовательской группы департамента инженерии биопроцессов и биотехнологий (DEBB), доктор философии в области генетической энзиматики, микробиологии и биоконверсии,

Куритиба.

Scopus Author ID: 7004252959.

Carlos Ricardo Soccol, Research Group Leader of Department of Bioprocesses Engineering and Biotechnology (DEBB),

Curitiba.

Scopus Author ID: 7004252959.

ЯньБинхуаYanBinghua

Бинхуа Янь, профессор, доктор философии,

Чанша.

Scopus Author ID: 56890254300.

Binghua Yan, PhD, Professor, 

Changsha.

Scopus Author ID: 56890254300.

https://orcid.org/0000-0003-0879-184XАвастиМукеш КумарAwasthiMukesh Kumar

Мукеш Кумар Авасти,  кандидат биологических наук, профессор, ученый-исследователь в области экологии и микробиологических технологий; пожизненный член Индийского общества биотехнологических исследований, Ассоциации научных конгрессов Индии, Общества фундаментальной и прикладной микологии, Ассоциации микробиологов, Национальной ассоциации по переработке твердых отходов Индии и Европейской федерации биотехнологий,

Янлин.

Scopus Author ID: 13003468000.

Mukesh Kumar Awasthi, PhD in Biological Sciences, A Life Member of the Indian Society for Biotechnology Research, the Association of Scientific Congresses of India, the Society for Fundamental and Applied Mycology, the Association of Microbiologists, the National Solid Waste Management Association of India, and the European Federation of Biotechnology,

Yangling.

Scopus Author ID: 13003468000.

https://orcid.org/0000-0002-6624-8936ТрчунянКаренTrchounianKaren

Карен Трчунян, доктор наук в области биофизики и биотехнологий, директор Научно-исследовательского института биологии, профессор кафедры биохимии, микробиологии и биотехнологии,

Ереван.

 Scopus Author ID: 23974981000.

Karen Trchounian, PhD in Biophysics and Biotechnology, Director at the Research Institute of Biology, Professor at the Department of Biochemistry, Microbiology, and Biotechnology,

Yerevan.

Scopus Author ID: 23974981000

https://orcid.org/0000-0002-5457-4603ЛиттиЮ. В.LittiYu. V.

Юрий Владимирович Литти, кандидат биологических наук, заведующий лабораторией микробиологии антропогенных мест обитания,

119071, г. Москва, Ленинский пр-т, д. 33, строение 2.

Researcher ID: C-4945-2014;

Scopus Author ID: 59312651000

Yuriy Vladimirovich Litti, Candidate of Biological Sciences, Head of Laboratory of Microbiology of Anthropogenic Habitats, 

119071, Moscow, Leninsky Prospekt, 33, Building 2.

Researcher ID: C-4945-2014; Scopus Author ID: 59312651000.

Институт микробиологии им. С. Н. Виноградского, Федеральный исследовательский центр «Фундаментальные основы биотехнологии» Российской академии наукРоссияS. N. Vinogradsky Institute of Microbiology, Federal Research Center «Fundamental Foundations of Biotechnology» of the Russian Academy of SciencesRussian FederationИнститут микробиологии им. С. Н. Виноградского, Федеральный исследовательский центр «Фундаментальные основы биотехнологии» Российской академии наук; Московский государственный университет имени М. В. Ломоносова, Биологический факультетРоссияS. N. Vinogradsky Institute of Microbiology, Federal Research Center «Fundamental Foundations of Biotechnology» of the Russian Academy of Sciences; Lomonosov Moscow State University, Faculty of BiologyRussian FederationФедеральное государственное бюджетное научное учреждение «Федеральный научный агроинженерный центр ВИМ»РоссияFederal State Budgetary Scientific Institution Federal Scientific Agroengineering Center VIMRussian FederationДурбанский технологический университет, Кафедра биотехнологии и пищевых технологий, факультет прикладных наукЮжно-Африканская РеспубликаDurban University of Technology, Department of Biotechnology and Food Technology, Faculty of Applied SciencesSouth AfricaФедеральный университет Параны, Политехнический центр, Кафедра биотехнологии и биоинженерииБразилияFederal University of Parana, Polytechnic Center, Department of Biotechnology and BioengineeringBrazilХунаньский сельскохозяйственный университет, Колледж окружающей среды и экологииКитайHunan Agricultural University, College of Environment and EcologyChinaСеверо-Западный университет A&F, Колледж природных ресурсов и окружающей средыКитайNorth-West A&F University, College of Natural Resources and EnvironmentChinaЕреванский государственный университет, Кафедра биохимии, микробиологии и биотехнологии биологического факультетаАрменияYerevan State University, Department of Biochemistry, Microbiology and Biotechnology, Faculty of BiologyArmenia20252301202601139106Copyright © Международный издательский дом научной периодики "Спейс, 20262026Международный издательский дом научной периодики "СпейсМеждународный издательский дом научной периодики "Спейсhttps://www.isjaee.com/jour/about/submissions#copyrightNoticehttps://www.isjaee.com/jour/article/view/2727

Биоводород, как перспективное биотопливо, обладает рядом ключевых преимуществ, которые включают нулевые выбросы углерода, экологическую устойчивость и высокую энергоэффективность. [NiFe]- и [FeFe]гидрогеназы играют ключевую роль в светонезависимом производстве биоводорода из биомассы с использованием микроорганизмов. Несмотря на значительный прогресс, по-прежнему существует множество проблем, препятствующих крупномасштабному промышленному внедрению этой технологии. В данном обзоре мы представляем подробный анализ структурных особенностей, распространенности и каталитических механизмов основных микроорганизмов, используемых в биореакторах темной ферментации и биоэлектрохимических системах. Мы также рассматриваем передовые стратегии повышения активности ферментов, такие как методы генной инженерии и инновационные методы оценки производительности. Кроме того, мы исследуем базовые механизмы, ответственные за увеличение производства газообразного водорода, включая перенос электронов, рост биомассы и синтез ферредоксина. В заключении обзора выделяются перспективные направления исследований, в частности, интеграция искусственного интеллекта в высокоэффективные системы производства биоводорода, которая может значительно ускорить прогресс в этой области.

Biohydrogen, a promising biofuel, has several key advantages, including zero carbon emissions, environmental sustainability, and high energy efficiency. [NiFe]- and [FeFe]-hydrogenases play a pivotal role in the light-independent production of biohydrogen from biomass using microorganisms. Despite significant progress, there are still numerous challenges that hinder large-scale industrial implementation of this technology. In this review, we provide a detailed analysis of the structural features, abundance, and catalytic mechanisms of key microorganisms used in dark fermentation bioreactors and bioelectrochemical systems. We also examine advanced strategies for enhancing enzyme activity, such as genetic engineering approaches and innovative performance evaluation techniques. Furthermore, we explore the underlying mechanisms responsible for increased hydrogen gas production, including electron transfer, biomass growth, and the synthesis of ferredoxin. The review concludes by emphasizing promising research directions, especially the integration of artificial intelligence into highly efficient biohydrogen production systems, which could significantly accelerate progress in this field.

биоводородтемновая ферментациягидрогеназадобавкигенетические модификациибиоэлектрохимическая системаbiohydrogendark fermentationhydrogenaseadditivesgenetic modificationsbioelectrochemical systemРабота частично поддержана Российским научным фондом, проект № 25-64-00027, https://rscf.ru/ project/25-64-00027/. Julio Cesar de Carvalho и Carlos Ricardo Soccol выражают благодарность Национальному совету по исследованиям и разработкам (CNPq) за финансирование, грант 440138/2022-1. Binghua Yan и Mukesh Kumar Awasthi выражают благодарность за финансовую поддержку в рамках проектов международного (регионального) сотрудничества и обмена Национального фонда естественных наук Китая (Inter organizations Collaborative Research-NSFC-BRICS, 52261145699). Santhosh Pillai выражает благодарность Национальному исследовательскому фонду (NRF) ЮАР за поддержку, грант № 150503.References. Sahil S., Singh R., Masakapalli S. K., Pareek N., Kovalev A. A., Litti Y. V. et al. 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The authors declare that there are no conflicts of interest present.