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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">alternative</journal-id><journal-title-group><journal-title xml:lang="ru">Альтернативная энергетика и экология (ISJAEE)</journal-title><trans-title-group xml:lang="en"><trans-title>Alternative Energy and Ecology (ISJAEE)</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1608-8298</issn><publisher><publisher-name>Международный издательский дом научной периодики "Спейс</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.15518/isjaee.2026.02.175-199</article-id><article-id custom-type="elpub" pub-id-type="custom">alternative-2785</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>VII. ЭКОЛОГИЧЕСКИЕ АСПЕКТЫ ЭНЕРГЕТИКИ. 17. Энергетика и экология. 17-8-0-0 Проблемы переработки промышленных и бытовых отходов</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>VII. ENVIRONMENTAL ASPECTS OF ENERGY. 17. Energy and ecology. 17-8-0-0 Problems of factory and domestic waste utilization</subject></subj-group></article-categories><title-group><article-title>Получение биоводорода из полисахарида, используемого для гидроразрыва пласта,  с использованием термофильных моно- и смешанных культур</article-title><trans-title-group xml:lang="en"><trans-title>Enhanced biohydrogen production from a fracturing fluid polysaccharide gel by thermophilic mono- and mixed cultures</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Потокина</surname><given-names>В. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Potokina</surname><given-names>V. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Потокина Виктория Вячеславовна, младший научный сотрудник лабораториимикробиологии антропогенных мест обитания</p><p>119071, г. Москва, Ленинский проспект, 33, стр. 2</p><p>Scopus ID: 57364304400</p></bio><bio xml:lang="en"><p>Potokina Victoriya Vyacheslavovna, Laboratory of Microbiology of Anthropogenic Habitat</p><p>119071, Moscow, Leninsky Prospekt, 33, Building 2</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-0085-3375</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Рыбкин</surname><given-names>Я. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Rybkin</surname><given-names>I. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Рыбкин Ярослав Андреевич, учёный-нефтехимик</p><p>117105, г. Москва, Варшавское шоссе, д. 9, стр. 1</p></bio><bio xml:lang="en"><p>Rybkin Iaroslav Andreevich, Petrochemical Scientist</p><p>117105, Moscow, Varshavskoe shosse, 9, Building 1B</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Паршина</surname><given-names>С. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Parshina</surname><given-names>S. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Паршина София Николаевна, старший научный сотрудник лаборатории микробиологии антропогенных мест обитания,кандидат биологических наук</p><p>119071, г. Москва, Ленинский проспект, 33, стр. 2</p><p>ResearcherID: A-8607-2014 Scopus ID: 35511680900</p></bio><bio xml:lang="en"><p>Parshina Sofia Nikolaevna, Laboratory of Microbiology of Anthropogenic Habitats, PhD</p><p>119071, Moscow, Leninsky Prospekt, 33, Building 2</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0006-0931-8789</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Иваненко</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Ivanenko</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Иваненко Артем Александрович, инженер лаборатории микробиологии антропогенных мест обитания, бакалавр</p><p>119071, г. Москва, Ленинский проспект, 33, стр. 2</p><p>Researcher ID: JAX-4154-2023</p><p>119899, г. Москва, Ленинские Горы, 1, стр. 12</p></bio><bio xml:lang="en"><p>Ivanenko Artem Alexandrovich, engineer in Laboratoryof Microbiology of Anthropogenic Habitats, bachelor</p><p>119071, Moscow, Leninsky Prospekt, 33, Building 2</p><p>119899, Moscow, Leninskie Gory, 1, Building 12 </p><p> </p></bio><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8215-2814</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Лайкова</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Laikova</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Лайкова Александра Алексеевна, младший научный сотрудник лаборатории микробиологии антропогенных мест обитания, аспирант</p><p>119071, г. Москва, Ленинский проспект, 33, стр. 2</p><p>Researcher ID: IVU-7977-2023 Scopus Author ID: 58044317600</p></bio><bio xml:lang="en"><p>Laikova Alexandra Alekseevna, junior researcher in Laboratory of Microbiology of Anthropogenic Habitats, PhD student</p><p>119071, Moscow, Leninsky Prospekt, 33, Building 2</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5525-0459</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Журавлева</surname><given-names>Е. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Zhuravleva</surname><given-names>E. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Журавлева Елена Александровна, науч. сотр. лаборатории микробиологии антропогенных мест обитания, канд. биол.наук</p><p>119071, г. Москва, Ленинский проспект, 33, стр. 2</p><p>Researcher ID: JBS-4297-2023 Scopus Author ID: 57216346570</p></bio><bio xml:lang="en"><p>Zhuravleva Elena Alexandrovna, researcher Laboratoryof Microbiology of Anthropogenic Habitats, postgraduate. PhD</p><p>119071, Moscow, Leninsky Prospekt, 33, Building 2</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-7458-0031</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шехурдина</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Shekhurdina</surname><given-names>S. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Шехурдина Светлана Витальевна, младший научный сотрудник лаборатории микробиологии антропогенных мест обитания, аспирант </p><p>119071, г. Москва, Ленинский проспект, 33, стр. 2</p><p>Scopus Author ID: 57564192200 Researcher ID: JZW-4863-2024</p></bio><bio xml:lang="en"><p>Shekhurdina Svetlana Vitalievna, unior researcher of Laboratory of Microbiology of Anthropogenic Habitats, PhD student</p><p>119071, Moscow, Leninsky Prospekt, 33, Building 2</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1983-3454</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ковалев</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Kovalev</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ковалев Андрей Александрович,</p><p>109428,. Москва, 1-й Институтский проезд, д. 5</p><p>Scopus Author ID: 57205285134</p></bio><bio xml:lang="en"><p>Kovalev Andrey Alexandrovich, chief researcher of the laboratory of bioenergy technologies, doctor of technical sciences</p><p>109428, Moscow, 1st Institutskiy Proezd, Building 5 </p></bio><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3603-3686</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ковалев</surname><given-names>Д. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Kovalev</surname><given-names>D. А.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ковалев Дмитрий Александрович, заведующий лабораторией биоэнергетических технологий, кандидат технических наук</p><p>109428,. Москва, 1-й Институтский проезд, д. 5</p><p>Researcher ID: K-4810-2015</p></bio><bio xml:lang="en"><p>Kovalev Dmitry Alexandrovich, head of the laboratory of bioenergy and supercritical technologies, candidate of technical sciences</p><p>109428, Moscow, 1st Institutskiy Proezd, Building 5 </p></bio><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Алахмари</surname><given-names>М.</given-names></name><name name-style="western" xml:lang="en"><surname>Alahmari</surname><given-names>M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Алахмари Манар, учёный-нефтехимик</p><p>34466, Дахран</p></bio><bio xml:lang="en"><p>Manar Alahmari, Petrochemical Scientist</p><p>34466, Dhahran</p></bio><xref ref-type="aff" rid="aff-5"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Орлов</surname><given-names>М. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Orlov</surname><given-names>M. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Орлов Максим Владимирович, Руководитель направления перспективных материалов</p><p>117105, г. Москва, Варшавское шоссе, д. 9, стр. 1</p></bio><bio xml:lang="en"><p>Orlov Maxim Vladimirovich, Program Director of Advanced Materials</p><p>117105, Moscow, Varshavskoe shosse, 9, Building 1B</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5457-4603</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Литти</surname><given-names>Ю. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Litti</surname><given-names>Y. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Литти Юрий Владимирович, заведующий лабораторией микробиологии антропогенных мест обитания, кандидатбиологических наук</p><p>119071, г. Москва, Ленинский проспект, 33, стр. 2</p><p>Researcher ID: C-4945-2014 Scopus Author ID: 59312651000</p></bio><bio xml:lang="en"><p>Litti Yuri Vladimirovich, Head of Laboratory of Microbiology of Anthropogenic Habitats, Candidate of Biological Sciences</p><p>119071, Moscow, Leninsky Prospekt, 33, Building 2</p></bio><email xlink:type="simple">litty-yuriy@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Институт микробиологии им. С. Н. Виноградского, ФИЦ Биотехнологии РАН</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal Research Center Fundamentals of Biotechnology of the Russian Academy of Sciences</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ООО «Арамко Инновейшнз»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Aramco Innovations LLC</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Институт микробиологии им. С. Н. Виноградского, ФИЦ Биотехнологии РАН; Московский государственный университет имени М. В. Ломоносова, биологический факультет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal Research Center Fundamentals of Biotechnology of the Russian Academy of Sciences; Department of Biology, Lomonosov Moscow State University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>Федеральное государственное бюджетное научное учреждение&#13;
«Федеральный научный агроинженерный центр ВИМ»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal Government Budgetary Institution of Science, «Federal Scientific Agroengeneering Сenter VIM»</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-5"><aff xml:lang="ru"><institution>Научно-исследовательский центр EXPEC «Сауди Арамко»</institution><country>Саудовская Аравия</country></aff><aff xml:lang="en"><institution>EXPEC Advanced Research Center Saudi Aramco</institution><country>Saudi Arabia</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2026</year></pub-date><pub-date pub-type="epub"><day>13</day><month>05</month><year>2026</year></pub-date><volume>0</volume><issue>2</issue><fpage>175</fpage><lpage>199</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Международный издательский дом научной периодики "Спейс, 2026</copyright-statement><copyright-year>2026</copyright-year><copyright-holder xml:lang="ru">Международный издательский дом научной периодики "Спейс</copyright-holder><copyright-holder xml:lang="en">Международный издательский дом научной периодики "Спейс</copyright-holder><license xlink:href="https://www.isjaee.com/jour/about/submissions#copyrightNotice" xlink:type="simple"><license-p>https://www.isjaee.com/jour/about/submissions#copyrightNotice</license-p></license></permissions><self-uri xlink:href="https://www.isjaee.com/jour/article/view/2785">https://www.isjaee.com/jour/article/view/2785</self-uri><abstract><p>Быстрый рост потребления воды при проведении операций гидроразрыва пласта (ГРП) требует разработки эффективных методов ее утилизации и повторного использования. Жидкости для ГРП обычно содержат органические полисахаридные гелеобразующие вещества, такие как гуаровая камедь и ее производные. В данной работе рассматривается темновая ферментация как многообещающий метод переработки полисахаридных отходов с получением водорода. Мы исследовали образование водорода из карбоксиметилгидроксипропилгуаровой камеди (CMHP-GG) и ее мономеров (галактозы и маннозы) с использованием термофильных штаммов бактерий Pseudoclostridium thermosuccinogenes AP-1, Tepidibaculum saccharolyticum AP-3, Thermohydrogenium kirishiense TR2, Thermoanaerobacter uzonensis 2K-2 и Thermoanaerobacterium thermosaccharolyticum SP-H2. Наибольшие выходы водорода из мономеров были получены для штаммов TR2 (77,9 мл из галактозы; 74,5 мл из маннозы) и SP-H2 (56,2 мл из галактозы; 43,1 мл из маннозы). При ферментации геля КМГП-ГК штамм AP-3 продемонстрировал самое высокое образование водорода как в монокультуре, так и в консорциумах со штаммами TR2, AP-1 и 2K-2 (40,1-37,5 мл) в соотношении 1:1. Для сравнения, консорциум с SP-H2 и смешанный консорциум всех штаммов образовали значительно меньше водорода (11,1 мл и 7,3 мл соответственно), по-видимому, из-за их метаболической несовместимости. Эти результаты подчеркивают потенциал подбора и применения микробных консорциумов для биоочистки жидкостей после гидроразрыва пласта с целью получения возобновляемого биоводорода и органических кислот.</p></abstract><trans-abstract xml:lang="en"><p>The rapid increase of water consumption in hydraulic fracturing (HF) operations, calls for creating effective disposal and water recycle methods. HF fluids often contain organic polysaccharide gelling agents like guar gum and its derivatives. This study explores dark fermentation as a promising method for converting this waste polymer into hydrogen, thereby enabling a waste-to-hydrogen valorization pathway. We determined hydrogen production from carboxymethylhydroxypropyl guar gum (CMHP-GG) and its monomers (galactose and mannose) using thermophilic bacterial strains Pseudoclostridium thermosuccinogenes AP-1, Tepidibaculum saccharolyticum AP-3, Thermohydrogenium kirishiense TR2, Thermoanaerobacter uzonensis 2K-2, and Thermoanaerobacterium thermosaccharolyticum SP-H2. The highest hydrogen yields from monomers were obtained by strains TR2 (77,9 mL from galactose; 74,5 mL from mannose) and SP-H2 (56,2 mL from galactose; 43,1 mL from mannose). During CMHP-GG gel fermentation, strain AP-3 demonstrated the highest hydrogen production, both in monoculture and in 1:1 consortia with strains TR2, AP-1, and 2K-2 (40,1-37,5 mL). In comparison, the consortium with SP-H2 and the mixed consortium of all strains yielded significantly less hydrogen (11,1 mL and 7,3 mL, respectively), due to metabolic incompatibility. These findings highlight the potential of specific microbial consortia for bio-refining post-fracturing fluids to produce renewable biohydrogen and organic acids.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>биоводород</kwd><kwd>темновая ферментация</kwd><kwd>Tepidibaculum saccharolyticum</kwd><kwd>жидкость для гидроразрыва пласта</kwd><kwd>микробный консорциум</kwd><kwd>карбоксиметилгидроксипропилгуаровая камедь (КМГП-ГК)</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Biohydrogen</kwd><kwd>Dark fermentation</kwd><kwd>Tepidibaculum saccharolyticum</kwd><kwd>Hydraulic fracturing fluid</kwd><kwd>Microbial consortium</kwd><kwd>Carboxymethylhydroxypropyl guar gum (CMHP-GG)</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">. Othman A., Aljawad M. S., Kalgaonkar R., Kamal M. S. 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