Оптимизация непрерывного двухстадийного производства биогитана из сточных вод кондитерской промышленности

Двухстадийная анаэробная ферментация представляет собой перспективный метод получения смеси Н₂ и СН₄ (биогитана) из различных органических отходов. Однако для масштабирования этого процесса требуется оптимизация условий, чтобы максимизировать удельный выход и скорость образования газообразных продуктов, а также обеспечить оптимальное соотношение Н₂ и СН₄ в биогитане. В данной работе варьировали гидравлическое время удержания (HRT) и нагрузку по органическому веществу (OLR) в мезофильном кислотогенном (RH) и термофильном метаногенном (R) реакторах с целью оптимизации непрерывного двухстадийного производства биогитана из сточных вод кондитерской промышленности. Удельный выход  Н₂ (87,8 ± 20,3 мл/г ХПК) и скорость образования Н₂ (2352 ± 376 мл/(л∙сут)) были максимальными при OLR в RH, равном 26,8 г ХПК/(л·сут). Удельный выход СН₄ (286,3 ± 29,1 мл/г ХПК) был максимален при HRT и OLR в R, равным 2 сут и 2,05 г ХПК/(л·сут), соответственно, а скорость образования СН₄ (964,6 ± 162 мл/(л·сут)) была максимальна при HRT и OLR в R, равным 2 сут и 4,6 г ХПК/(л·сут), соответственно. Оптимальный состав биогитана (10% Н₂ , 50,4% СН₄ , 39,6% СО₂ ) был получен, когда RH функционировал при OLR, равным  12,8 г ХПК/(л·сут), а R при HRT и OLR, равным 3 сут и 1,37 г ХПК/(л·сут), соответственно. В оптимальных режимах работы в Н₂-продуцирующем микробном сообществе RH доминировали Caproiciproducens и Clostridium sensu stricto 12, а в R – синтрофные бактерии Cloacimonadaceae W5, Lentimicrobium, Anaerolinea, и гидрогенотрофные метаногены Methanothermobacter. Полученные результаты способствуют дальнейшему совершенствованию двухстадийной анаэробной ферментации для производства биогитана из концентрированных сточных вод.

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