Получение биоводорода из полисахарида, используемого для гидроразрыва пласта, с использованием термофильных моно- и смешанных культур

Быстрый рост потребления воды при проведении операций гидроразрыва пласта (ГРП) требует разработки эффективных методов ее утилизации и повторного использования. Жидкости для ГРП обычно содержат органические полисахаридные гелеобразующие вещества, такие как гуаровая камедь и ее производные. В данной работе рассматривается темновая ферментация как многообещающий метод переработки полисахаридных отходов с получением водорода. Мы исследовали образование водорода из карбоксиметилгидроксипропилгуаровой камеди (CMHP-GG) и ее мономеров (галактозы и маннозы) с использованием термофильных штаммов бактерий Pseudoclostridium thermosuccinogenes AP-1, Tepidibaculum saccharolyticum AP-3, Thermohydrogenium kirishiense TR2, Thermoanaerobacter uzonensis 2K-2 и Thermoanaerobacterium thermosaccharolyticum SP-H2. Наибольшие выходы водорода из мономеров были получены для штаммов TR2 (77,9 мл из галактозы; 74,5 мл из маннозы) и SP-H2 (56,2 мл из галактозы; 43,1 мл из маннозы). При ферментации геля КМГП-ГК штамм AP-3 продемонстрировал самое высокое образование водорода как в монокультуре, так и в консорциумах со штаммами TR2, AP-1 и 2K-2 (40,1-37,5 мл) в соотношении 1:1. Для сравнения, консорциум с SP-H2 и смешанный консорциум всех штаммов образовали значительно меньше водорода (11,1 мл и 7,3 мл соответственно), по-видимому, из-за их метаболической несовместимости. Эти результаты подчеркивают потенциал подбора и применения микробных консорциумов для биоочистки жидкостей после гидроразрыва пласта с целью получения возобновляемого биоводорода и органических кислот.

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