COMPARATIVE STUDY OF ARTICLES PRODUCED FROM TITANIUM AND NITINOL POWDERS BY SELECTIVE LASER SINTERING (SLS) AS POROUS SCAFFOLDS FOR STEM CELLS

The potential of porous implants synthesized from titanium or NiTi (known as - nitinol) for the creation of scaffold tissue constructions (carrier matrix) are discussed. Sufficient understanding of the nature of laser synthesized titanium and nitinol structures was developed to determine their suitability for being used as functional implants. This resulted in superior tissue-to-implant fixation and development of minimal invasive surgical procedures. The role of surface roughness of the porous matrix on cell morphology, proliferation and adhesion are discussed. Test porous samples were prepared by using the Selective Laser Synthesis (SLS) method with different kinds of surface treatments. Surface microstructure and roughness were analyzed by Scanning Electron and Optical Microscopy. Studies were carried out on the ingress of primary cultures of dermal fibroblasts and mesenchymal stromal human cells into the porous structures from 1-2 days to three months of culture. A study of bone intergrowth in the porous carrier matrix made from nitinol and pure titanium was determined in vitro. The results reveal the influence of surface roughness on cell proliferation, morphology and adhesion. Pure titanium was well tolerated by the living cells, but the number of focal contacts was lower than for the nitinol samples.

селективное лазерное спекание (СЛС), титан, нитинол, клеточные каркасы, стволовые клетки, функциональные имплантаты, морфология, пролиферация, адгезия, клеточные фибробласты, мезенхимальные стромальные клетки человека, костные ткани, in vitro, selective laser sintering (SLS), titanium, nitinol, cell scaffolds, stem cells, functional implants, morphology, proliferation, adhesion, cell fibroblasts, mesenchymal stromal cells of human, bone tissues, in vitro

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